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Title: Compositions and methods for detecting gene rearrangements and translocations

Patent ·
OSTI ID:873254
 [1];  [1]
  1. Chicago, IL
+ Show Author Affiliations

Disclosed is a series of nucleic acid probes for use in diagnosing and monitoring certain types of leukemia using, e.g., Southern and Northern blot analyses and fluorescence in situ hybridization (FISH). These probes detect rearrangements, such as translocations involving chromosome band 11q23 with other chromosomes bands, including 4q21, 6q27, 9p22, 19p13.3, in both dividing leukemic cells and interphase nuclei. The breakpoints in all such translocations are clustered within an 8.3 kb BamHI genomic region of the MLL gene. A novel 0.7 kb BamH1 cDNA fragment derived from this gene detects rearrangements on Southern blot analysis with a single BamHI restriction digest in all patients with the common 11q23 translocations and in patients with other 11q23 anomalies. Northern blot analyses are presented demonstrating that the MLL gene has multiple transcripts and that transcript size differentiates leukemic cells from normal cells. Also disclosed are MLL fusion proteins, MLL protein domains and anti-MLL antibodies.

Research Organization:
Argonne National Laboratory (ANL), Argonne, IL (United States)
DOE Contract Number:
FG02-86ER60408
Assignee:
Arch Development Corp. (Chicago, IL)
Patent Number(s):
US 6121419
OSTI ID:
873254
Country of Publication:
United States
Language:
English

References (6)

Cloning of ELL, a gene that fuses to MLL in a t(11;19)(q23;p13.1) in acute myeloid leukemia. journal December 1994
Rearrangement of the MLL Gene in Acute Lymphoblastic and Acute Myeloid Leukemias with 11q23 Chromosomal Translocations journal September 1993
The t(4;11) chromosome translocation of human acute leukemias fuses the ALL-1 gene, related to Drosophila trithorax, to the AF-4 gene journal November 1992
Rearrangements of the MLL gene in therapy-related acute myeloid leukemia in patients previously treated with agents targeting DNA- topoisomerase II journal December 1993
11q23 translocations split the "AT-hook" cruciform DNA-binding region and the transcriptional repression domain from the activation domain of the mixed-lineage leukemia (MLL) gene. journal October 1994
The trithorax gene, a trans-acting regulator of the bithorax complex in Drosophila, encodes a protein with zinc-binding domains. journal March 1990

Similar Records

Cloning of ELL, a gene that fuses to MLL in a t(11; 19)(q23; p13. 1) in acute myeloid leukemia
Journal Article · Tue Dec 06 00:00:00 EST 1994 · Proceedings of the National Academy of Sciences of the United States of America; (United States) · OSTI ID:873254
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Cloning of the chromosome translocation breakpoint junction of the t(14; 19) in chronic lymphocytic leukemia
Journal Article · Tue Dec 01 00:00:00 EST 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States) · OSTI ID:873254
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11q23 Translocations split the [open quotes]AT-hook[close quotes] cruciform DNA-binding region and the transcriptional repression domain from the activation domain of the mixed-lineage leukemia (MLL) gene
Journal Article · Tue Oct 25 00:00:00 EDT 1994 · Proceedings of the National Academy of Sciences of the United States of America; (United States) · OSTI ID:873254

Related Subjects

compositions
methods
detecting
rearrangements
translocations
disclosed
series
nucleic
acid
probes
diagnosing
monitoring
types
leukemia
southern
northern
blot
analyses
fluorescence
situ
hybridization
fish
detect
involving
chromosome
band
11q23
chromosomes
bands
including
4q21
6q27
9p22
19p13
dividing
leukemic
cells
interphase
nuclei
breakpoints
clustered
kb
bamhi
genomic
region
mll
novel
bamh1
cdna
fragment
derived
detects
analysis
single
restriction
digest
patients
common
anomalies
demonstrating
multiple
transcripts
transcript
size
differentiates
normal
fusion
proteins
protein
domains
anti-mll
antibodies
normal cells
acid probes
nucleic acid
acid probe
situ hybridization
dna fragment
blot analyses
fusion protein
fusion proteins
interphase nuclei
probes detect
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