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Title: Reducing nontemplated 3' nucleotide addition to polynucleotide transcripts

Patent ·
OSTI ID:873102
 [1]
  1. Bloomington, IN
+ Show Author Affiliations

Non-template 3' nucleotide addition to a transcript is reduced by transcribing a transcript from a template comprising an ultimate and/or penultimate 5' ribose having a C'2 substituent such as methoxy, which reduces non-template 3' nucleotide addition to the transcript. The methods are shown to be applicable to a wide variety of polymerases, including Taq, T7 RNA polymerase, etc.

Research Organization:
Univ. of California (United States)
DOE Contract Number:
FG03-86ER60406
Assignee:
Regents of University of California (Oakland, CA); Advanced Research and Technology, Inc. (Indianapolis, IN)
Patent Number(s):
US 6090590
OSTI ID:
873102
Country of Publication:
United States
Language:
English

References (2)

Non-hydrogen bonding 'terminator' nucleosides increase the 3'-end homogeneity of enzymatic RNA and DNA synthesis journal June 1996
Substrate properties of C′-methyl UTP derivatives in T7 RNA polymerase reactions. Evidence for N-type NTP conformation journal January 1997

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Related Subjects

reducing
nontemplated
nucleotide
addition
polynucleotide
transcripts
non-template
transcript
reduced
transcribing
template
comprising
ultimate
penultimate
ribose
substituent
methoxy
reduces
methods
shown
applicable
wide
variety
polymerases
including
taq
t7
rna
polymerase
etc
rna polymerase
t7 rna
wide variety
nucleotide addition
rna poly
/435/999/