skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: cDNA encoding a polypeptide including a hev ein sequence

Abstract

A cDNA clone (HEV1) encoding hevein was isolated via polymerase chain reaction (PCR) using mixed oligonucleotides corresponding to two regions of hevein as primers and a Hevea brasiliensis latex cDNA library as a template. HEV1 is 1018 nucleotides long and includes an open reading frame of 204 amino acids. The deduced amino acid sequence contains a putative signal sequence of 17 amino acid residues followed by a 187 amino acid polypeptide. The amino-terminal region (43 amino acids) is identical to hevein and shows homology to several chitin-binding proteins and to the amino-termini of wound-induced genes in potato and poplar. The carboxyl-terminal portion of the polypeptide (144 amino acids) is 74-79% homologous to the carboxyl-terminal region of wound-inducible genes of potato. Wounding, as well as application of the plant hormones abscisic acid and ethylene, resulted in accumulation of hevein transcripts in leaves, stems and latex, but not in roots, as shown by using the cDNA as a probe. A fusion protein was produced in E. coli from the protein of the present invention and maltose binding protein produced by the E. coli.

Inventors:
 [1];  [2];  [3];  [4]
  1. Okemos, MI
  2. Dilbeek, BE
  3. Scarsdale, NY
  4. New York, NY
Publication Date:
Research Org.:
Michigan State Univ., East Lansing, MI (United States)
OSTI Identifier:
873070
Patent Number(s):
US 6083687
Application Number:
07/888,367
Assignee:
Board of Trustees operating Michigan State University (East Lansing, MI)
DOE Contract Number:  
AC02-76ER01338
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
cdna; encoding; polypeptide; including; sequence; clone; hev1; hevein; isolated; via; polymerase; chain; reaction; pcr; mixed; oligonucleotides; corresponding; regions; primers; hevea; brasiliensis; latex; library; template; 1018; nucleotides; reading; frame; 204; amino; acids; deduced; acid; contains; putative; signal; 17; residues; followed; 187; amino-terminal; region; 43; identical; homology; chitin-binding; proteins; amino-termini; wound-induced; genes; potato; poplar; carboxyl-terminal; portion; 144; 74-79; homologous; wound-inducible; wounding; application; plant; hormones; abscisic; ethylene; resulted; accumulation; transcripts; leaves; stems; roots; shown; probe; fusion; protein; produced; coli; maltose; binding; cdna clone; deduced amino; reading frame; chain reaction; binding protein; acid sequence; amino acid; polymerase chain; amino acids; cdna library; dna encoding; cdna encoding; residues followed; protein produced; putative signal; wound-induced genes; wound-inducible genes; abscisic acid; carboxyl-terminal portion; carboxyl-terminal region; maltose binding; plant hormones; chitin-binding proteins; amino-terminal region; sequence contains; signal sequence; oligonucleotides corresponding; mixed oligonucleotides; via polymerase; encoding hevein; hevea brasiliensis; acid residues; acid residue; acid polypeptide; brasiliensis latex; latex cdna; isolated via; polypeptide including; fusion protein; hormones abscisic; hevein transcripts; nucleotides corresponding; /435/530/

Citation Formats

Raikhel, Natasha V, Broekaert, Willem F, Chua, Nam-Hai, and Kush, Anil. cDNA encoding a polypeptide including a hev ein sequence. United States: N. p., 2000. Web.
Raikhel, Natasha V, Broekaert, Willem F, Chua, Nam-Hai, & Kush, Anil. cDNA encoding a polypeptide including a hev ein sequence. United States.
Raikhel, Natasha V, Broekaert, Willem F, Chua, Nam-Hai, and Kush, Anil. 2000. "cDNA encoding a polypeptide including a hev ein sequence". United States. https://www.osti.gov/servlets/purl/873070.
@article{osti_873070,
title = {cDNA encoding a polypeptide including a hev ein sequence},
author = {Raikhel, Natasha V and Broekaert, Willem F and Chua, Nam-Hai and Kush, Anil},
abstractNote = {A cDNA clone (HEV1) encoding hevein was isolated via polymerase chain reaction (PCR) using mixed oligonucleotides corresponding to two regions of hevein as primers and a Hevea brasiliensis latex cDNA library as a template. HEV1 is 1018 nucleotides long and includes an open reading frame of 204 amino acids. The deduced amino acid sequence contains a putative signal sequence of 17 amino acid residues followed by a 187 amino acid polypeptide. The amino-terminal region (43 amino acids) is identical to hevein and shows homology to several chitin-binding proteins and to the amino-termini of wound-induced genes in potato and poplar. The carboxyl-terminal portion of the polypeptide (144 amino acids) is 74-79% homologous to the carboxyl-terminal region of wound-inducible genes of potato. Wounding, as well as application of the plant hormones abscisic acid and ethylene, resulted in accumulation of hevein transcripts in leaves, stems and latex, but not in roots, as shown by using the cDNA as a probe. A fusion protein was produced in E. coli from the protein of the present invention and maltose binding protein produced by the E. coli.},
doi = {},
url = {https://www.osti.gov/biblio/873070}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jul 04 00:00:00 EDT 2000},
month = {Tue Jul 04 00:00:00 EDT 2000}
}

Works referenced in this record:

Improvement of the dideoxy chain termination method of DNA sequencing by use of deoxy-7-deazaguanosine triphosphate in place of dGTP
journal, January 1986


Comparison of some molecular, enzymatic and antifungal properties of chitinases from thorn-apple, tobacco and wheat
journal, November 1988


Primary structure of wheat germ agglutinin isolectin 2. Peptide order deduced from x-ray structure
journal, January 1984


Systemic accumulation of specific mRNAs in response to wounding in poplar trees.
journal, October 1989


Host-Pathogen Interactions: XVIII. ISOLATION AND BIOLOGICAL ACTIVITY OF GLYCINOL, A PTEROCARPAN PHYTOALEXIN SYNTHESIZED BY SOYBEANS
journal, August 1981


Ethylene Biosynthesis and its Regulation in Higher Plants
journal, June 1984


Ethylene-regulated gene expression: molecular cloning of the genes encoding an endochitinase from Phaseolus vulgaris.
journal, September 1986


Co- and post-translational processing of the hevein preproprotein of latex of the rubber tree (Hevea brasiliensis)
journal, August 1991


Cloning and Characterization of Root-Specific Barley Lectin
journal, September 1989


DNA sequencing with chain-terminating inhibitors
journal, December 1977


Plastid-localised seed acyl-carrier protein of Brassica napus is encoded by a distinct, nuclear multigene family
journal, June 1988


Regulation of wound ethylene synthesis in plants
journal, July 1980


A simple and very efficient method for generating cDNA libraries
journal, November 1983


Isolation of cDNA clones coding for spinach nitrite reductase: Complete sequence and nitrate induction
journal, April 1988


Sequence complexity of polyadenylated ribonucleic acid from soybean suspension culture cells
journal, June 1979


A rapid and sensitive assay for chitinase using tritiated chitin
journal, December 1977


Plant chitinases are potent inhibitors of fungal growth
journal, November 1986


Regulation of synthesis of proteinase inhibitors I and II mRNAs in leaves of wounded tomato plants
journal, November 1986


Chitinase cDNA Cloning and mRNA Induction by Fungal Elicitor, Wounding, and Infection
journal, January 1988


Cell-type-specific expression of a wheat-germ agglutinin gene in embryos and young seedlings of Triticum aestivum
journal, December 1988


A Chitin-Binding Lectin from Stinging Nettle Rhizomes with Antifungal Properties
journal, September 1989


Abscisic acid is involved in the wound-induced expression of the proteinase inhibitor II gene in potato and tomato
journal, December 1989


Extensive homologies between lectins from non-leguminous plants
journal, January 1986


Homology between chitinases that are induced by TMV infection of tobacco
journal, January 1987


Effect of Ethylene Action Inhibitors upon Wound-Induced Gene Expression in Tomato Pericarp
journal, September 1989


Wound-induced accumulation of mRNA containing a hevein sequence in laticifers of rubber tree (Hevea brasiliensis).
journal, October 1990


Plant defense genes are regulated by ethylene
journal, August 1987