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Title: In vitro gastrointestinal mimetic protocol for measuring bioavailable contaminants

Patent ·
OSTI ID:872923
 [1]
  1. Berkeley, CA
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The present invention relates to measurements of contaminants in the soil and other organic or environmental materials, using a biologically relevant chemical analysis that will measure the amount of contaminants in a given sample that may be expected to be absorbed by a human being ingesting the contaminated soil. According to the present invention, environmental samples to be tested are added to a pre-prepared physiological composition of bile salts and lipids. They are thoroughly mixed and then the resulting mixture is separated e.g. by centrifugation. The supernatant is then analyzed for the presence of contaminants and these concentrations are compared to the level of contaminants in the untreated samples. It is important that the bile salts and lipids be thoroughly pre-mixed to form micelles.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
DOE Contract Number:
AC03-76SF00098
Assignee:
Regents of University of California (Oakland, CA)
Patent Number(s):
US 6040188
OSTI ID:
872923
Country of Publication:
United States
Language:
English

References (10)

Soil adsorption alters kinetics and bioavailability of benzene in orally exposed male rats journal March 1988
A Multicompartmental Dynamic Computer-controlled Model Simulating the Stomach and Small Intestine journal March 1995
Modulating effects in human diets of dietary fibre and beef, and of time and dose on the reactive microcapsule trapping of benzo[ a ]pyrene matabolites in the rat gastrointestinal tract journal January 1990
Development of an in vitro screening test to evaluate the in vivo bioaccessibility of ingested mine-waste lead journal December 1993
Estimation of Lead and Arsenic Bioavailability Using a Physiologically Based Extraction Test journal January 1996
In vitro simulation of gastric digestion of milk proteins: comparison between in vitro and in vivo data journal February 1992
Physical-chemical behavior of dietary and biliary lipids during intestinal digestion and absorption. 2. Phase analysis and aggregation states of luminal lipids during duodenal fat digestion in healthy adult human beings journal February 1990
Micromineralogy of mine wastes in relation to lead bioavailability, Butte, Montana journal July 1993
Estimation of Agitation Intensity in the GI Tract in Humans and Dogs Based on in Vitro/in Vivo Correlation journal January 1995
Physical-chemical behavior of dietary and biliary lipids during intestinal digestion and absorption. 1. Phase behavior and aggregation states of model lipid systems patterned after aqueous duodenal contents of healthy adult human beings journal February 1990

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vitro
gastrointestinal
mimetic
protocol
measuring
bioavailable
contaminants
relates
measurements
soil
organic
environmental
materials
biologically
relevant
chemical
analysis
measure
amount
sample
expected
absorbed
human
ingesting
contaminated
according
samples
tested
added
pre-prepared
physiological
composition
bile
salts
lipids
thoroughly
mixed
resulting
mixture
separated
centrifugation
supernatant
analyzed
presence
concentrations
compared
level
untreated
pre-mixed
form
micelles
environmental sample
contaminated soil
chemical analysis
resulting mixture
environmental samples
treated sample
thoroughly mix
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