Invasive cleavage of nucleic acids
Abstract
The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
- Inventors:
-
- Madison, WI
- Publication Date:
- Research Org.:
- Third Wave Technologies, Inc., Madison, WI (United States)
- OSTI Identifier:
- 872670
- Patent Number(s):
- US 5985557
- Assignee:
- Third Wave Technologies, Inc. (WI)
- DOE Contract Number:
- FG02-94ER81891
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- invasive; cleavage; nucleic; acids; relates; means; detection; characterization; acid; sequences; variations; methods; forming; structure; target; sequence; cleaving; site-specific; manner; structure-specific; nuclease; activity; variety; enzymes; cleave; target-dependent; indicating; presence; specific; cleavage structure; acid sequence; nucleic acid; acid sequences; nucleic acids; target sequence; acid cleavage; specific nucleic; structure-specific nuclease; specific variations; site-specific manner; nuclease activity; target-dependent cleavage; specific manner; /435/999/
Citation Formats
Prudent, James R, Hall, Jeff G, Lyamichev, Victor I, Brow, Mary Ann D, and Dahlberg, James E. Invasive cleavage of nucleic acids. United States: N. p., 1999.
Web.
Prudent, James R, Hall, Jeff G, Lyamichev, Victor I, Brow, Mary Ann D, & Dahlberg, James E. Invasive cleavage of nucleic acids. United States.
Prudent, James R, Hall, Jeff G, Lyamichev, Victor I, Brow, Mary Ann D, and Dahlberg, James E. 1999.
"Invasive cleavage of nucleic acids". United States. https://www.osti.gov/servlets/purl/872670.
@article{osti_872670,
title = {Invasive cleavage of nucleic acids},
author = {Prudent, James R and Hall, Jeff G and Lyamichev, Victor I and Brow, Mary Ann D and Dahlberg, James E},
abstractNote = {The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.},
doi = {},
url = {https://www.osti.gov/biblio/872670},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 1999},
month = {Fri Jan 01 00:00:00 EST 1999}
}
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