Modified Acyl-ACP desaturase

Patent · Tue Mar 30 00:00:00 EST 1999

OSTI ID:872227

Cahoon, Edgar B ^[1]; Shanklin, John ^[1]; Lindqvist, Ylva ^[2]; Schneider, Gunter ^[2]

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1. Shoreham, NY
2. Jarfalla, SE

Disclosed is a method for modifying the chain length and double bond positional specificities of a soluble plant fatty acid desaturase. More specifically, the method involves modifying amino acid contact residues in the substrate binding channel of the soluble fatty acid desaturase which contact the fatty acid. Specifically disclosed is the modification of an acyl-ACP desaturase. Amino acid contact residues which lie within the substrate binding channel are identified, and subsequently replaced with different residues to effect the modification of activity.

• Research Organization: Associated Universities, Inc., Upton, NY (United States)
• DOE Contract Number: AC02-76CH00016
• Assignee: Brookhaven Science Associates LLC (Upton, NY)
• Patent Number(s): US 5888790
• Application Number: 08/853979
• OSTI ID: 872227
• Country of Publication: United States
• Language: English

Related Subjects

modified
acyl-acp
desaturase
disclosed
method
modifying
chain
length
double
bond
positional
specificities
soluble
plant
fatty
acid
specifically
involves
amino
contact
residues
substrate
binding
channel
modification
lie
identified
subsequently
replaced
effect
activity
plant fatty
acid contact
specifically disclosed
method involves
amino acid
fatty acid
chain length
contact residues
acid desaturase
acyl-acp desaturase
modified acyl-acp
method involve
modifying amino
involves modifying
soluble plant
positional specificities
bond positional
double bond
/435/