skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Cloning and expression of the gene for bacteriophage T7 RNA polymerase

Patent ·
OSTI ID:872140
 [1];  [2];  [3];  [4];  [5]
  1. Stony Brook, NY
  2. Basel, CH
  3. Setauket, NY
  4. Waterloo, CA
  5. Bellport, NY
+ Show Author Affiliations

This application describes a means to clone a functional gene for bacteriophage T7 RNA polymerase. Active T7 RNA polymerase is produced from the cloned gene, and a plasmid has been constructed that can produce the active enzyme in large amounts. T7 RNA polymerase transcribes DNA very efficiently and is highly selective for a relatively long promoter sequence. This enzyme is useful for synthesizing large amounts of RNA in vivo or in vitro, and is capable of producing a single RNA selectively from a complex mixture of DNAs. The procedure used to obtain a clone of the R7 RNA polymerase gene can be applied to other T7-like phages to obtain clones that produce RNA polymerases having different promoter specificities, different bacterial hosts, or other desirable properties. T7 RNA polymerase is also used in a system for selective, high-level synthesis of RNAs and proteins in suitable host cells.

Research Organization:
Associated Universities, Inc., Upton, NY (United States)
DOE Contract Number:
AC02-76CH00016
Assignee:
Brookhaven Science Associates LLC (Upton, NY)
Patent Number(s):
US 5869320
Application Number:
08/784,201
OSTI ID:
872140
Country of Publication:
United States
Language:
English

References (6)

Cloning and expression of the gene for bacteriophage T7 RNA polymerase. journal April 1984
Organization and Expression of Bacteriophage T7 DNA journal January 1983
Bacteriophage T7 DNA replication in vitro. Stimulation of DNA synthesis by T7 RNA polymerase. journal August 1980
Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements journal June 1983
Nucleotide sequence of the gene for bacteriophage T7 RNA polymerase journal February 1984
Bacteriophage SP6-specific RNA polymerase. I. Isolation and characterization of the enzyme. journal May 1982

Similar Records

Related Subjects

cloning
expression
bacteriophage
t7
rna
polymerase
application
describes
means
clone
functional
active
produced
cloned
plasmid
constructed
produce
enzyme
amounts
transcribes
dna
efficiently
highly
selective
relatively
promoter
sequence
useful
synthesizing
vivo
vitro
capable
producing
single
selectively
complex
mixture
dnas
procedure
obtain
r7
applied
t7-like
phages
clones
polymerases
specificities
bacterial
hosts
desirable
properties
high-level
synthesis
rnas
proteins
suitable
host
cells
bacteriophage t7
bacterial host
application describes
suitable host
rna polymerase
host cells
t7 rna
highly selective
rna polymerases
desirable properties
host cell
promoter specificities
promoter sequence
rna selectively
t7-like phages
single rna
active t7
bacterial hosts
produce rna
polymerase transcribes
active enzyme
obtain clones
complex mixture
transcribes dna
high-level synthesis
rna poly
/435/530/999/