Cloning and expression of autogenes encoding RNA polymerases of T7-like bacteriophages

Patent · Thu Jan 01 00:00:00 EST 1998

OSTI ID:871951

Studier, F William ^[1]; Dubendorff, John W ^[2]

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1. Stony Brook, NY
2. Sound Beach, NY

This invention relates to the cloning and expression of autogenes encoding RNA polymerases of T7 and T7-like bacteriophages, in which the RNA polymerase gene is transcribed from a promoter which is recognized by the encoded RNA polymerase. Cloning of T7 autogenes was achieved by reducing the activity of the RNA polymerase sufficiently to permit host cell growth. T7 RNA polymerase activity was controlled by combining two independent methods: lac-repression of the recombinant lac operator-T7 promoter in the autogene and inhibition of the polymerase by T7 lysozyme. Expression systems for producing the RNA polymerases of T7 and other T7-like bacteriophages, and expression systems for producing selected gene products are described, as well as other related materials and methods.

• Research Organization: Brookhaven National Laboratory (BNL), Upton, NY (United States)
• Assignee: Associated Universities, Inc. (Washington, DC)
• Patent Number(s): US 5830694
• OSTI ID: 871951
• Country of Publication: United States
• Language: English

Related Subjects

cloning
expression
autogenes
encoding
rna
polymerases
t7-like
bacteriophages
relates
t7
polymerase
transcribed
promoter
recognized
encoded
achieved
reducing
activity
sufficiently
permit
host
cell
growth
controlled
combining
independent
methods
lac-repression
recombinant
operator-t7
autogene
inhibition
lysozyme
systems
producing
selected
products
described
related
materials
t7-like bacteriophages
expression systems
cell growth
related materials
rna polymerase
t7 rna
rna polymerases
host cell
producing selected
encoding rna
autogenes encoding
genes encoding
rna poly
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