Method of manufacturing a matrix for the detection of mismatches

Patent · Thu Jan 01 00:00:00 EST 1998

OSTI ID:871647

Ershov, Gennady Moiseevich ^[1]; Mirzabekov, Andrei Darievich ^[1]

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1. Moscow, RU

This method for preparing micromatrices consists in applying a specially-patterned intermediate layer of laser-absorbing substance on a solid support. The configuration of the sublayer fully corresponds to the topology of the manufactured matrix. The intermediate layer is further covered by a continuous layer of gel , the gel and the material of the support being transparent towards laser radiation. The gel layer is irradiated by a laser beam for a time needed to evaporate simultaneously the gel in the places immediately above the laser-absorbing sublayer and the sublayer itself. Oligonucleotides from a chosen set are then attached to the formed gel `cells`, one oligonucleotide to each cell. This method is intended for use in biotechnology, specifically for deciphering the nucleotide sequence of DNA.

• Research Organization: Argonne National Laboratory (ANL), Argonne, IL (United States)
• Assignee: University of Chicago (Chicago, IL)
• Patent Number(s): US 5770721
• OSTI ID: 871647
• Country of Publication: United States
• Language: English

Related Subjects

method
manufacturing
matrix
detection
mismatches
preparing
micromatrices
consists
applying
specially-patterned
intermediate
layer
laser-absorbing
substance
solid
support
configuration
sublayer
corresponds
topology
manufactured
covered
continuous
material
transparent
towards
laser
radiation
irradiated
beam
time
evaporate
simultaneously
immediately
oligonucleotides
chosen
set
attached
formed
cells
oligonucleotide
cell
intended
biotechnology
specifically
deciphering
nucleotide
sequence
dna
solid support
nucleotide sequence
laser beam
laser radiation
intermediate layer
continuous layer
/999/216/264/422/435/