Cytoplasmic bacteriophage display system
Abstract
Disclosed are display vectors comprising DNA encoding a portion of a structural protein from a cytoplasmic bacteriophage, joined covalently to a protein or peptide of interest. Exemplified are display vectors wherein the structural protein is the T7 bacteriophage capsid protein. More specifically, in the exemplified display vectors the C-terminal amino acid residue of the portion of the capsid protein is joined to the N-terminal residue of the protein or peptide of interest. The portion of the T7 capsid protein exemplified comprises an N-terminal portion corresponding to form 10B of the T7 capsid protein. The display vectors are useful for high copy number display or lower copy number display (with larger fusion). Compositions of the type described herein are useful in connection with methods for producing a virus displaying a protein or peptide of interest.
- Inventors:
-
- Stony Brook, NY
- Setauket, NY
- Publication Date:
- Research Org.:
- Associated Universities, Inc., Upton, NY (United States)
- OSTI Identifier:
- 871626
- Patent Number(s):
- US 5766905
- Application Number:
- 08/664,449
- Assignee:
- Associated Universities Inc. (Washington, DC)
- DOE Contract Number:
- AC02-76CH00016
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- cytoplasmic; bacteriophage; display; disclosed; vectors; comprising; dna; encoding; portion; structural; protein; joined; covalently; peptide; exemplified; t7; capsid; specifically; c-terminal; amino; acid; residue; n-terminal; comprises; corresponding; form; 10b; useful; copy; larger; fusion; compositions; type; described; connection; methods; producing; virus; displaying; amino acid; dna encoding; type described; display vectors; acid residue; structural protein; cytoplasmic bacteriophage; /435/424/
Citation Formats
Studier, F William, and Rosenberg, Alan H. Cytoplasmic bacteriophage display system. United States: N. p., 1998.
Web.
Studier, F William, & Rosenberg, Alan H. Cytoplasmic bacteriophage display system. United States.
Studier, F William, and Rosenberg, Alan H. 1998.
"Cytoplasmic bacteriophage display system". United States. https://www.osti.gov/servlets/purl/871626.
@article{osti_871626,
title = {Cytoplasmic bacteriophage display system},
author = {Studier, F William and Rosenberg, Alan H},
abstractNote = {Disclosed are display vectors comprising DNA encoding a portion of a structural protein from a cytoplasmic bacteriophage, joined covalently to a protein or peptide of interest. Exemplified are display vectors wherein the structural protein is the T7 bacteriophage capsid protein. More specifically, in the exemplified display vectors the C-terminal amino acid residue of the portion of the capsid protein is joined to the N-terminal residue of the protein or peptide of interest. The portion of the T7 capsid protein exemplified comprises an N-terminal portion corresponding to form 10B of the T7 capsid protein. The display vectors are useful for high copy number display or lower copy number display (with larger fusion). Compositions of the type described herein are useful in connection with methods for producing a virus displaying a protein or peptide of interest.},
doi = {},
url = {https://www.osti.gov/biblio/871626},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jun 16 00:00:00 EDT 1998},
month = {Tue Jun 16 00:00:00 EDT 1998}
}
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