skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Method of producing a cellulase-containing cell-free fermentate produced from microorganism ATCC 55702

Patent ·
OSTI ID:871571

Bacteria which produce large amounts of cellulose-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques.

Research Organization:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
DOE Contract Number:
AC05-84OR21400
Assignee:
Lockheed Martin Energy Systems, Inc. (Oak Ridge, TN)
Patent Number(s):
US 5756337
OSTI ID:
871571
Country of Publication:
United States
Language:
English

References (29)

Characterization of an Endoglucanase from Pseudomonas fluorescens subsp. cellulosa Produced in Escherichia coli and Regulation of the Expression of Its Cloned Gene journal January 1988
Specificity of an esterase (XYLD) from Pseudomonas fluorescens subsp. cellulosa journal February 1995
Purification and Properties of Extracellular and Cell-bound Cellulase Components of Pseudomonas fluorescens var. cellulosa journal January 1970
Partial characterization of an inducible amylase produced byPseudomonas cellulosa: Scientific note journal March 1995
The non-catalytic cellulose-binding domain of a novel cellulase from Pseudomonas fluorescens subsp. cellulosa is important for the efficient hydrolysis of Avicel journal August 1995
Biogenesis of Multiple Cellulase Components of Pseudomonas fluorescens var. cellulosa journal March 1974
Purification ofPseudomonas fluorescens subsp.cellulosa endoglucanases produced inEscherichia coli journal May 1990
β-Glucosidase, β-galactosidase, family A cellulases, family F xylanases and two barley glycanases form a superfamily of enzymes wit 8-fold β/α architecture and with two conserved glutamates near the carboxy-terminal ends of β-strands four and seven journal April 1995
Characterization of the cellulose-degrading bacterium NCIMB 10462 journal September 1995
Evidence for multiple carboxymethylcellulase genes in Pseudomonas fluorescens subsp. cellulosa journal January 1987
Intracellular Distribution and Some Properties of β -Glucosidases of a Cellulolytic Pseudomonad journal November 1976
Characterization and expression in Escherichia coli of an endoglucanase gene of Pseudomonas fluorescens subsp. cellulosa journal July 1988
Localization of Cellulase Components in Pseudomonas fluorescens var. cellulosa* journal January 1971
Characterization of the gene celD and its encoded product 1,4-β-d-glucan glucohydrolase D from Pseudomonas fluorescens subsp. cellulosa journal August 1992
Production of highly thermostable xylanase by a wild strain of thermophilic fungus Thermoascus aurantiacus and partial characterization of the enzyme journal September 1994
The cellodextrinase from Pseudomonas fluorescens subsp. cellulosa consists of multiple functional domains journal November 1991
A modular esterase from Pseudomonas fluorescens subsp. cellulosa contains a non-catalytic cellulose-binding domain journal September 1993
The resistance of cellulases and xylanases to proteolytic inactivation journal April 1995
Molecular Cloning of Multiple Xylanase Genes from Pseudomonas fluorescens subsp. cellulosa journal December 1988
Partial characterization ofPseudomonas fluorescens subsp.cellulosa endoglucanase activity produced inEscherichia coli journal April 1990
The N-terminal region of an endoglucanase from Pseudomonas fluorescens subspecies cellulosa constitutes a cellulose-binding domain that is distinct from the catalytic centre journal May 1990
DNA sequence analysis of endoglucanase genes fromPseudomonas fluorescens subsp.cellulosa andPseudomonas sp. NCIB 8634 journal December 1990
Pseudomonas fluorescens subsp. cellulosa : an alternative model for bacterial cellulase journal March 1992
Intracellular distribution and some properties of .BETA.-glucosidases of a cellulolytic pseudomonad. journal January 1976
Isolation of Endoglucanase Genes from Pseudomonas fluorescens subsp. cellulosa and a Pseudomonas sp journal January 1986
Xylanase B and an arabinofuranosidase from Pseudomonas fluorescens subsp. cellulosa contain identical cellulose-binding domains and are encoded by adjacent genes journal December 1990
Effect of Nature and Supply of Carbon Sources on Cellulase Formation in Pseudomonas fluorescens var. cellulosa journal January 1970
Use of cellulase fromPseudomonas fluorescens for the hydrolysis of waste paper in an attrition bioreactor: Scientific note journal September 1995
Expression of an endoglucanase gene of Pseudomonas fluorescens var. cellulosa in Zymomonas mobilis journal March 1988