Hybrid slab-microchannel gel electrophoresis system

Patent · Thu Jan 01 00:00:00 EST 1998

OSTI ID:871521

Balch, Joseph W ^[1]; Carrano, Anthony V ^[1]; Davidson, James C ^[1]; Koo, Jackson C ^[2]

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1. Livermore, CA
2. San Ramon, CA

A hybrid slab-microchannel gel electrophoresis system. The hybrid system permits the fabrication of isolated microchannels for biomolecule separations without imposing the constraint of a totally sealed system. The hybrid system is reusable and ultimately much simpler and less costly to manufacture than a closed channel plate system. The hybrid system incorporates a microslab portion of the separation medium above the microchannels, thus at least substantially reducing the possibility of non-uniform field distribution and breakdown due to uncontrollable leakage. A microslab of the sieving matrix is built into the system by using plastic spacer materials and is used to uniformly couple the top plate with the bottom microchannel plate.

• Research Organization: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
• DOE Contract Number: W-7405-ENG-48
• Assignee: Regents Of University Of California (Oakland, CA)
• Patent Number(s): US 5746901
• OSTI ID: 871521
• Country of Publication: United States
• Language: English

Related Subjects

hybrid
slab-microchannel
electrophoresis
permits
fabrication
isolated
microchannels
biomolecule
separations
imposing
constraint
totally
sealed
reusable
ultimately
simpler
costly
manufacture
closed
channel
plate
incorporates
microslab
portion
separation
medium
substantially
reducing
possibility
non-uniform
field
distribution
breakdown
due
uncontrollable
leakage
sieving
matrix
built
plastic
spacer
materials
uniformly
couple
top
bottom
microchannel
channel plate
microchannel plate
top plate
substantially reducing
spacer material
uniform field
hybrid slab-microchannel
field distribution
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