Procedure for normalization of cDNA libraries

Patent · Wed Jan 01 00:00:00 EST 1997

OSTI ID:871292

Bonaldo, Maria DeFatima ^[1]; Soares, Marcelo Bento ^[1]

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1. New York, NY

This invention provides a method to normalize a cDNA library constructed in a vector capable of being converted to single-stranded circles and capable of producing complementary nucleic acid molecules to the single-stranded circles comprising: (a) converting the cDNA library in single-stranded circles; (b) generating complementary nucleic acid molecules to the single-stranded circles; (c) hybridizing the single-stranded circles converted in step (a) with complementary nucleic acid molecules of step (b) to produce partial duplexes to an appropriate Cot; (e) separating the unhybridized single-stranded circles from the hybridized single-stranded circles, thereby generating a normalized cDNA library.

• Research Organization: Columbia Univ., New York, NY (United States)
• DOE Contract Number: FG02-91ER61233
• Assignee: Trustees of Columbia University in City of New York (New York, NY)
• Patent Number(s): US 5702898
• OSTI ID: 871292
• Country of Publication: United States
• Language: English

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provides
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converted
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circles
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complementary
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generating
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partial
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normalized cdna
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appropriate cot
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/435/999/