Capillaries for use in a multiplexed capillary electrophoresis system

Yeung, Edward S; Chang, Huan-Tsang; Fung, Eliza N

Title: Capillaries for use in a multiplexed capillary electrophoresis system

Patent · Wed Jan 01 00:00:00 EST 1997

OSTI ID:871267

Yeung, Edward S ^[1]; Chang, Huan-Tsang ^[2]; Fung, Eliza N ^[1]

Ames, IA
Silver Spring, MD

The invention provides a side-entry optical excitation geometry for use in a multiplexed capillary electrophoresis system. A charge-injection device is optically coupled to capillaries in the array such that the interior of a capillary is imaged onto only one pixel. In Sanger-type 4-label DNA sequencing reactions, nucleotide identification ("base calling") is improved by using two long-pass filters to split fluorescence emission into two emission channels. A binary poly(ethyleneoxide) matrix is used in the electrophoretic separations.

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Research Organization:: Ames Laboratory (AMES), Ames, IA; Iowa State Univ., Ames, IA (United States)

DOE Contract Number:: W-7405-ENG-82

Assignee:: Iowa State University Research Foundation (Ames, IA)

Patent Number(s):: US 5695626

Application Number:: 08/667,950

OSTI ID:: 871267

Country of Publication:: United States

Language:: English

References (47)

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capillaries
multiplexed
capillary
electrophoresis
provides
side-entry
optical
excitation
geometry
charge-injection
device
optically
coupled
array
interior
imaged
pixel
sanger-type
4-label
dna
sequencing
reactions
nucleotide
identification
base
calling
improved
long-pass
filters
split
fluorescence
emission
channels
binary
poly
ethyleneoxide
matrix
electrophoretic
separations
multiplexed capillary
pass filters
capillary electrophoresis
dna sequencing
optically coupled
fluorescence emission
pass filter
sequencing reactions
side-entry optical
split fluorescence
base calling
binary poly
long-pass filters
optical excitation
nucleotide identification
charge-injection device
electrophoretic separations
injection device
emission channels
excitation geometry
electrophoretic separation
sequencing reaction
/204/

Title: Capillaries for use in a multiplexed capillary electrophoresis system

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