High density array fabrication and readout method for a fiber optic biosensor

Patent · Wed Jan 01 00:00:00 EST 1997

OSTI ID:871246

Pinkel, Daniel ^[1]; Gray, Joe ^[2]

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1. Walnut Creek, CA
2. San Francisco, CA

The invention relates to the fabrication and use of biosensors comprising a plurality of optical fibers each fiber having attached to its "sensor end" biological "binding partners" (molecules that specifically bind other molecules to form a binding complex such as antibody-antigen, lectin-carbohydrate, nucleic acid-nucleic acid, biotin-avidin, etc.). The biosensor preferably bears two or more different species of biological binding partner. The sensor is fabricated by providing a plurality of groups of optical fibers. Each group is treated as a batch to attach a different species of biological binding partner to the sensor ends of the fibers comprising that bundle. Each fiber, or group of fibers within a bundle, may be uniquely identified so that the fibers, or group of fibers, when later combined in an array of different fibers, can be discretely addressed. Fibers or groups of fibers are then selected and discretely separated from different bundles. The discretely separated fibers are then combined at their sensor ends to produce a high density sensor array of fibers capable of assaying simultaneously the binding of components of a test sample to the various binding partners on the different fibers of the sensor array. The transmission ends of the optical fibers are then discretely addressed to detectors--such as a multiplicity of optical sensors. An optical signal, produced by binding of the binding partner to its substrate to form a binding complex, is conducted through the optical fiber or group of fibers to a detector for each discrete test. By examining the addressed transmission ends of fibers, or groups of fibers, the addressed transmission ends can transmit unique patterns assisting in rapid sample identification by the sensor.

• Research Organization: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
• DOE Contract Number: AC03-76SF00098
• Assignee: Regents of University of California (Oakland, CA)
• Patent Number(s): US 5690894
• OSTI ID: 871246
• Country of Publication: United States
• Language: English

Related Subjects

density
array
fabrication
readout
method
fiber
optic
biosensor
relates
biosensors
comprising
plurality
optical
fibers
attached
sensor
biological
binding
partners
molecules
specifically
bind
form
complex
antibody-antigen
lectin-carbohydrate
nucleic
acid-nucleic
acid
biotin-avidin
etc
preferably
bears
species
partner
fabricated
providing
treated
batch
attach
bundle
uniquely
identified
combined
discretely
addressed
selected
separated
bundles
produce
capable
assaying
simultaneously
components
sample
various
transmission
detectors-such
multiplicity
sensors
signal
produced
substrate
conducted
detector
discrete
examining
transmit
unique
patterns
assisting
rapid
identification
binding partner
binding partners
sensor array
optical sensors
optical fibers
fiber optic
nucleic acid
optical fiber
optical signal
optical sensor
specifically bind
readout method
optic biosensor
density array
array fabrication
biosensors comprising
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