Method for in situ or ex situ bioremediation of hexavalent chromium contaminated soils and/or groundwater

Patent · Tue Oct 28 00:00:00 EST 1997

OSTI ID:871209

Turick, Charles E ^[1]; Apel, William W ^[1]

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1. Idaho Falls, ID

A method of reducing the concentration of Cr(VI) in a liquid aqueous residue comprises the steps of providing anaerobic Cr(VI) reducing bacteria, mixing the liquid aqueous residue with a nutrient medium to form a mixture, and contacting the mixture with the anaerobic Cr(VI) reducing bacteria such that Cr(VI) is reduced to Cr(III). The anaerobic Cr(VI) reducing bacteria appear to be ubiquitous in soil and can be selected by collecting a soil sample, diluting the soil sample with a sterile diluent to form a diluted sample, mixing the diluted sample with an effective amount of a nutrient medium and an effective amount of Cr(VI) to form a mixture, and incubating the mixture in the substantial absence of oxygen such that growth of Cr(VI) sensitive microorganisms is inhibited and growth of the anaerobic Cr(VI) reducing bacteria is stimulated. A method of in situ bioremediation of Cr(VI) contaminated soil and/or groundwater is also disclosed.

• Research Organization: Idaho National Laboratory (INL), Idaho Falls, ID (United States)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC07-94ID13223; AC07-76ID01570
• Assignee: Lockheed Idaho Technologies Company (Idaho Falls, ID)
• Patent Number(s): US 5681739
• Application Number: 08/452591
• OSTI ID: 871209
• Country of Publication: United States
• Language: English

Related Subjects

method
situ
bioremediation
hexavalent
chromium
contaminated
soils
groundwater
reducing
concentration
liquid
aqueous
residue
comprises
steps
providing
anaerobic
bacteria
mixing
nutrient
medium
form
mixture
contacting
reduced
iii
appear
ubiquitous
soil
selected
collecting
sample
diluting
sterile
diluent
diluted
effective
amount
incubating
substantial
absence
oxygen
growth
sensitive
microorganisms
inhibited
stimulated
disclosed
reducing bacteria
nutrient medium
contaminated soils
liquid aqueous
contaminated soil
soil sample
effective amount
situ bioremediation
substantial absence
hexavalent chromium
aqueous residue
/435/