Method for nucleic acid hybridization using single-stranded DNA binding protein

Tabor, Stanley; Richardson, Charles C

Title: Method for nucleic acid hybridization using single-stranded DNA binding protein

Patent · Mon Jan 01 00:00:00 EST 1996

OSTI ID:870501

Tabor, Stanley ^[1]; Richardson, Charles C ^[2]

Cambridge, MA
Chestnut Hill, MA

Method of nucleic acid hybridization for detecting the presence of a specific nucleic acid sequence in a population of different nucleic acid sequences using a nucleic acid probe. The nucleic acid probe hybridizes with the specific nucleic acid sequence but not with other nucleic acid sequences in the population. The method includes contacting a sample (potentially including the nucleic acid sequence) with the nucleic acid probe under hybridizing conditions in the presence of a single-stranded DNA binding protein provided in an amount which stimulates renaturation of a dilute solution (i.e., one in which the t.sub.1/2 of renaturation is longer than 3 weeks) of single-stranded DNA greater than 500 fold (i.e., to a t.sub.1/2 less than 60 min, preferably less than 5 min, and most preferably about 1 min.) in the absence of nucleotide triphosphates.

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Research Organization:: Harvard University

DOE Contract Number:: FG02-88ER60688

Assignee:: President and Fellows of Harvard College (Cambridge, MA)

Patent Number(s):: US 5534407

OSTI ID:: 870501

Country of Publication:: United States

Language:: English

References (25)

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Genetic Recombination of Bacteriophage T7 DNA in Vitro Sadowski, Paul D.; Bradley, William; Lee, Donald Mechanistic Studies of DNA Replication and Genetic Recombination https://doi.org/10.1016/B978-0-12-048850-6.50085-5	book	January 1980
Requirements for primer synthesis by bacteriophage T7 63-kDa gene 4 protein. Roles of template sequence and T7 56-kDa gene 4 protein. Mendelman, L. V.; Richardson, C. C. Journal of Biological Chemistry, Vol. 266, Issue 34 https://doi.org/10.1016/S0021-9258(18)54488-4	journal	December 1991
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Purification and Characterization of a DNA-pairing and Strand Transfer Activity from Mitotic Saccharomyces cerevisiae Halbrook, J.; McEntee, K. Journal of Biological Chemistry, Vol. 264, Issue 35 https://doi.org/10.1016/S0021-9258(19)30095-X	journal	December 1989
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method
nucleic
acid
hybridization
single-stranded
dna
binding
protein
detecting
presence
specific
sequence
population
sequences
probe
hybridizes
contacting
sample
potentially
including
hybridizing
conditions
provided
amount
stimulates
renaturation
dilute
solution
weeks
500
fold
60
preferably
absence
nucleotide
triphosphates
stranded dna
acid hybridization
dilute solution
binding protein
acid sequence
nucleic acid
acid sequences
acid probe
specific nucleic
single-stranded dna
dna binding
/435/

Title: Method for nucleic acid hybridization using single-stranded DNA binding protein

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References (25)

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