Method for rapid base sequencing in DNA and RNA with two base labeling

Patent · Sun Jan 01 00:00:00 EST 1995

OSTI ID:869830

Jett, James H ^[1]; Keller, Richard A ^[1]; Martin, John C ^[1]; Posner, Richard G ^[1]; Marrone, Babetta L ^[1]; Hammond, Mark L ^[1]; Simpson, Daniel J ^[1]

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1. Los Alamos, NM

Method for rapid-base sequencing in DNA and RNA with two-base labeling and employing fluorescent detection of single molecules at two wavelengths. Bases modified to accept fluorescent labels are used to replicate a single DNA or RNA strand to be sequenced. The bases are then sequentially cleaved from the replicated strand, excited with a chosen spectrum of electromagnetic radiation, and the fluorescence from individual, tagged bases detected in the order of cleavage from the strand.

• Research Organization: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
• DOE Contract Number: W-7405-ENG-36
• Assignee: Regents of University of California Office of Technology Transfer (Alameda, CA)
• Patent Number(s): US 5405747
• OSTI ID: 869830
• Country of Publication: United States
• Language: English

Related Subjects

method
rapid
base
sequencing
dna
rna
labeling
rapid-base
two-base
employing
fluorescent
detection
single
molecules
wavelengths
bases
modified
accept
labels
replicate
strand
sequenced
sequentially
cleaved
replicated
excited
chosen
spectrum
electromagnetic
radiation
fluorescence
individual
tagged
detected
cleavage
electromagnetic radiation
rapid base
base sequencing
fluorescent labels
single molecule
fluorescent detection
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