Self-referencing spectrophotometric measurements

Patent · Sat Jan 01 00:00:00 EST 1994

OSTI ID:869214

O'Rourke, Patrick E ^[1]; Van Hare, David R ^[2]

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1. Martinez, GA
2. Aiken, SC

A method for measuring the concentration of a chemical substance by spectrophotometry comprising the steps of placing a sample of a photoreactive substance between the light source and a spectrophotometer, obtaining an absorption spectrum of the substance using a fixed amount of light from the light source, obtaining a second absorption spectrum after a short interval, comparing the two to determine the concentration of the chemical substance from the difference in the spectra. If the chemical substance is not photoreactive, a photoreactive mixture can be made with a photoreactive dye that has photoreactive properties unique to the mixture. Alternatively, an optically transparent substrate can absorb the substance or the dye/substance mixture.

• Research Organization: E I DUPONT DE NEMOURS & CO INC
• DOE Contract Number: AC09-76SR00001
• Assignee: United States Department of Energy (Washington, DC)
• Patent Number(s): US 5298428
• OSTI ID: 869214
• Country of Publication: United States
• Language: English

Related Subjects

self-referencing
spectrophotometric
measurements
method
measuring
concentration
chemical
substance
spectrophotometry
comprising
steps
placing
sample
photoreactive
light
source
spectrophotometer
obtaining
absorption
spectrum
fixed
amount
interval
comparing
determine
difference
spectra
mixture
dye
properties
unique
alternatively
optically
transparent
substrate
absorb
transparent substrate
spectrophotometric measurements
optically transparent
light source
absorption spectrum
chemical substance
parent substrate
active substance
/436/356/422/