Thermostable purified endoglucanas from acidothermus cellulolyticus ATCC 43068

Himmel, Michael E; Adney, William S; Tucker, Melvin P; Grohmann, Karel

Title: Thermostable purified endoglucanas from acidothermus cellulolyticus ATCC 43068

Patent · Sat Jan 01 00:00:00 EST 1994

OSTI ID:869113

Himmel, Michael E ^[1]; Adney, William S ^[2]; Tucker, Melvin P ^[3]; Grohmann, Karel ^[4]

Littleton, CO
Golden, CO
Lakewood, CO
Winter Haven, FL

A purified low molecular weight cellulase endoglucanase I having a molecular weight of between about 57,420 to about 74,580 daltons from Acidothermus cellulolyticus (ATCC 43068). The cellulase is water soluble, possesses both C.sub.1 and C.sub.x types of enzyme activity, a high degree of stability toward heat, and exhibits optimum temperature activity at about 83.degree. C. at pH's from about 2 to about 9, and in inactivation temperature of about 110.degree. C. at pH's from about 2 to about 9.

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Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States); Midwest Research Institute, Kansas City, MO (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC02-83CH10093

Assignee:: Midwest Research Institute (Kansas City, MO)

Patent Number(s):: US 5275944

OSTI ID:: 869113

Country of Publication:: United States

Language:: English

References (6)

Bacterium produces thermostable cellulase Seltze, Richard Chemical & Engineering News Archive, Vol. 65, Issue 18 https://doi.org/10.1021/cen-v065n018.p023	journal	May 1987
Ultra-Thermostable Cellulases From Acidothermus cellulolyticus: Comparison of Temperature Optima with Previously Reported Cellulases Tucker, Melvin P.; Mohagheghi, Ali; Grohmann, Karel Nature Biotechnology, Vol. 7, Issue 8 https://doi.org/10.1038/nbt0889-817	journal	August 1989
The Production of Cellulases Mandels, Mary; Weber, James Advances in Chemistry https://doi.org/10.1021/ba-1969-0095.ch023	book	June 1969
Cellulolytic enzyme system of Acetivibrio cellulolyticus Saddler, J. N.; Khan, A. W. Canadian Journal of Microbiology, Vol. 27, Issue 3 https://doi.org/10.1139/m81-045	journal	March 1981
Mechanisms of Thermophily Amelunxen, Remi E.; Murdock, Archie L.; Welker, Neil E. CRC Critical Reviews in Microbiology, Vol. 6, Issue 4 https://doi.org/10.3109/10408417809090626	journal	January 1978
Isolation and Characterization of Acidothermus cellulolyticus gen. nov., sp. nov., a New Genus of Thermophilic, Acidophilic, Cellulolytic Bacteria MOHAGHEGHI, A.; GROHMANN, K.; HIMMEL, M. International Journal of Systematic Bacteriology, Vol. 36, Issue 3, p. 435-443 https://doi.org/10.1099/00207713-36-3-435	journal	July 1986

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Related Subjects

thermostable
purified
endoglucanas
acidothermus
cellulolyticus
atcc
43068
molecular
weight
cellulase
endoglucanase
57
420
74
580
daltons
water
soluble
possesses
types
enzyme
activity
degree
stability
heat
exhibits
optimum
temperature
83
ph
inactivation
110
cellulolyticus atcc
optimum temperature
enzyme activity
water soluble
molecular weight
acidothermus cellulolyticus
thermostable purified
temperature activity
weight cellulase
inactivation temperature
purified endoglucanas
/435/

Title: Thermostable purified endoglucanas from acidothermus cellulolyticus ATCC 43068

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