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Title: Phase-sensitive flow cytometer

Abstract

A phase-sensitive flow cytometer (FCM) provides additional FCM capability to use the fluorescence lifetime of one or more fluorochromes bound to single cells to provide additional information regarding the cells. The resulting fluorescence emission can be resolved into individual fluorescence signals if two fluorochromes are present or can be converted directly to a decay lifetime from a single fluorochrome. The excitation light for the fluorochromes is modulated to produce an amplitude modulated fluorescence pulse as the fluorochrome is excited in the FCM. The modulation signal also forms a reference signal that is phase-shifted a selected amount for subsequent mixing with the output modulated fluorescence intensity signal in phase-sensitive detection circuitry. The output from the phase-sensitive circuitry is then an individual resolved fluorochrome signal or a single fluorochrome decay lifetime, depending on the applied phase shifts.

Inventors:
 [1]
  1. Los Alamos, NM
Publication Date:
Research Org.:
Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
OSTI Identifier:
869071
Patent Number(s):
US 5270548
Assignee:
United States of America as represented by United States (Washington, DC)
DOE Contract Number:  
W-7405-ENG-36
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
phase-sensitive; flow; cytometer; fcm; provides; additional; capability; fluorescence; lifetime; fluorochromes; bound; single; cells; provide; information; regarding; resulting; emission; resolved; individual; signals; converted; directly; decay; fluorochrome; excitation; light; modulated; produce; amplitude; pulse; excited; modulation; signal; forms; reference; phase-shifted; selected; amount; subsequent; mixing; output; intensity; detection; circuitry; depending; applied; phase; shifts; provides additional; amplitude modulated; phase-sensitive detection; sensitive detection; modulation signal; provide additional; phase shift; reference signal; excitation light; flow cytometer; fluorescence emission; fluorescence intensity; detection circuitry; phase shifts; single cell; detection circuit; information regarding; fluorescence lifetime; selected amount; phase-sensitive flow; converted directly; additional information; /250/

Citation Formats

Steinkamp, John A. Phase-sensitive flow cytometer. United States: N. p., 1993. Web.
Steinkamp, John A. Phase-sensitive flow cytometer. United States.
Steinkamp, John A. 1993. "Phase-sensitive flow cytometer". United States. https://www.osti.gov/servlets/purl/869071.
@article{osti_869071,
title = {Phase-sensitive flow cytometer},
author = {Steinkamp, John A},
abstractNote = {A phase-sensitive flow cytometer (FCM) provides additional FCM capability to use the fluorescence lifetime of one or more fluorochromes bound to single cells to provide additional information regarding the cells. The resulting fluorescence emission can be resolved into individual fluorescence signals if two fluorochromes are present or can be converted directly to a decay lifetime from a single fluorochrome. The excitation light for the fluorochromes is modulated to produce an amplitude modulated fluorescence pulse as the fluorochrome is excited in the FCM. The modulation signal also forms a reference signal that is phase-shifted a selected amount for subsequent mixing with the output modulated fluorescence intensity signal in phase-sensitive detection circuitry. The output from the phase-sensitive circuitry is then an individual resolved fluorochrome signal or a single fluorochrome decay lifetime, depending on the applied phase shifts.},
doi = {},
url = {https://www.osti.gov/biblio/869071}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 1993},
month = {Fri Jan 01 00:00:00 EST 1993}
}

Works referenced in this record:

Phase-Resolved Fluorescence Spectroscopy
journal, November 1984


Two-color immunofluorescence using a fluorescence-activated cell sorter.
journal, July 1977


Improved multilaser/multiparameter flow cytometer for analysis and sorting of cells and particles
journal, November 1991


The Measurement and Analysis of Heterogeneous Emissions by Multifrequency Phase and Modulation Fluorometry
journal, January 1984