Rotor assembly and assay method

Patent · Fri Jan 01 00:00:00 EST 1993

OSTI ID:868912

Burtis, Carl A ^[1]; Johnson, Wayne F ^[2]; Walker, William A ^[3]

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1. Oak Ridge, TN
2. Loudon, TN
3. Knoxville, TN

A rotor assembly for carrying out an assay includes a rotor body which is rotatable about an axis of rotation, and has a central chamber and first, second, third, fourth, fifth, and sixth chambers which are in communication with and radiate from the central chamber. The rotor assembly further includes a shuttle which is movable through the central chamber and insertable into any of the chambers, the shuttle including a reaction cup carrying an immobilized antigen or an antibody for transport among the chambers. A method for carrying out an assay using the rotor assembly includes moving the reaction cup among the six chambers by passing the cup through the central chamber between centrifugation steps in order to perform the steps of: separating plasma from blood cells, binding plasma antibody or antigen, washing, drying, binding enzyme conjugate, reacting with enzyme substrate and optically comparing the resulting reaction product with unreacted enzyme substrate solution. The movement of the reaction cup can be provided by attaching a magnet to the reaction cup and supplying a moving magnetic field to the rotor.

• Research Organization: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
• DOE Contract Number: AC05-84OR21400
• Assignee: Martin Marietta Energy Systems, Inc. (Oak Ridge, TN)
• Patent Number(s): US 5242803
• OSTI ID: 868912
• Country of Publication: United States
• Language: English

Related Subjects

rotor
assembly
assay
method
carrying
rotatable
axis
rotation
central
chamber
third
fourth
fifth
sixth
chambers
communication
radiate
shuttle
movable
insertable
including
reaction
cup
immobilized
antigen
antibody
transport
moving
six
passing
centrifugation
steps
perform
separating
plasma
blood
cells
binding
washing
drying
enzyme
conjugate
reacting
substrate
optically
comparing
resulting
product
unreacted
solution
movement
provided
attaching
magnet
supplying
magnetic
field
central chamber
rotor assembly
blood cells
magnetic field
reaction product
resulting reaction
blood cell
assay method
/435/422/436/