Enzyme electrochemical sensor electrode and method of making it

Patent · Wed Jan 01 00:00:00 EST 1992

OSTI ID:868138

Rishpon, Judith ^[1]; Zawodzinski, Thomas A ^[1]; Gottesfeld, Shimshon ^[1]

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1. Los Alamos, NM

An electrochemical sensor electrode is formed from an electronic conductor coated with a casting solution containing a perfluorosulfonic acid ionomer and a selected enzyme. The selected enzyme catalyzes a reaction between a predetermined substance in a solution and oxygen to form an electrochemically active compound that is detected at the electronic conductor. The resulting perfluorosulfonic acid polymer provides a stable matrix for the enzyme for long lived enzyme activity, wherein only thin coatings are required on the metal conductor. The polymer also advantageously repels interfering substances from contacting the enzyme and contains quantities of oxygen to maintain a sensing capability during conditions of oxygen depletion in the sample. In one particular embodiment, glucose oxidase is mixed with the perfluorosulfonic acid ionomer to form an electrode for glucose detection.

• Research Organization: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
• DOE Contract Number: W-7405-ENG-36
• Assignee: United States of America as represented by Department of Energy (Washington, DC)
• Patent Number(s): US 5082550
• OSTI ID: 868138
• Country of Publication: United States
• Language: English

Related Subjects

enzyme
electrochemical
sensor
electrode
method
formed
electronic
conductor
coated
casting
solution
containing
perfluorosulfonic
acid
ionomer
selected
catalyzes
reaction
predetermined
substance
oxygen
form
electrochemically
active
compound
detected
resulting
polymer
provides
stable
matrix
lived
activity
coatings
required
metal
advantageously
repels
interfering
substances
contacting
contains
quantities
maintain
sensing
capability
conditions
depletion
sample
particular
embodiment
glucose
oxidase
mixed
detection
acid polymer
perfluorosulfonic acid
enzyme activity
sensor electrode
particular embodiment
electrochemical sensor
sulfonic acid
chemical sensor
metal conductor
solution containing
electronic conductor
casting solution
electrochemically active
acid ionomer
polymer provides
chemically active
active compound
selected enzyme
enzyme catalyzes
metal conduct
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