Method for rapid base sequencing in DNA and RNA

Patent · Mon Jan 01 00:00:00 EST 1990

OSTI ID:867551

Jett, James H ^[1]; Keller, Richard A ^[1]; Martin, John C ^[1]; Moyzis, Robert K ^[1]; Ratliff, Robert L ^[1]; Shera, E Brooks ^[1]; Stewart, Carleton C ^[1]

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1. Los Alamos, NM

A method is provided for the rapid base sequencing of DNA or RNA fragments wherein a single fragment of DNA or RNA is provided with identifiable bases and suspended in a moving flow stream. An exonuclease sequentially cleaves individual bases from the end of the suspended fragment. The moving flow stream maintains the cleaved bases in an orderly train for subsequent detection and identification. In a particular embodiment, individual bases forming the DNA or RNA fragments are individually tagged with a characteristic fluorescent dye. The train of bases is then excited to fluorescence with an output spectrum characteristic of the individual bases. Accordingly, the base sequence of the original DNA or RNA fragment can be reconstructed.

• Research Organization: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
• DOE Contract Number: W-7405-ENG-36
• Assignee: United States of America (Washington, DC)
• Patent Number(s): US 4962037
• OSTI ID: 867551
• Country of Publication: United States
• Language: English

Related Subjects

method
rapid
base
sequencing
dna
rna
provided
fragments
single
fragment
identifiable
bases
suspended
moving
flow
stream
exonuclease
sequentially
cleaves
individual
maintains
cleaved
orderly
train
subsequent
detection
identification
particular
embodiment
forming
individually
tagged
characteristic
fluorescent
dye
excited
fluorescence
output
spectrum
accordingly
sequence
original
reconstructed
output spectrum
particular embodiment
base sequence
flow stream
fluorescent dye
rapid base
base sequencing
subsequent detection
/435/436/