Size-exclusion chromatography system for macromolecular interaction analysis
- Downers Grove, IL
A low pressure, microcomputer controlled system employing high performance liquid chromatography (HPLC) allows for precise analysis of the interaction of two reversibly associating macromolecules such as proteins. Since a macromolecular complex migrates faster than its components during size-exclusion chromatography, the difference between the elution profile of a mixture of two macromolecules and the summation of the elution profiles of the two components provides a quantifiable indication of the degree of molecular interaction. This delta profile is used to qualitatively reveal the presence or absence of significant interaction or to rank the relative degree of interaction in comparing samples and, in combination with a computer simulation, is further used to quantify the magnitude of the interaction in an arrangement wherein a microcomputer is coupled to analytical instrumentation in a novel manner.
- Research Organization:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- DOE Contract Number:
- W-31109-ENG-38
- Assignee:
- United States of America as represented by United States (Washington, DC)
- Patent Number(s):
- US 4762617
- OSTI ID:
- 866670
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
chromatography
macromolecular
interaction
analysis
pressure
microcomputer
controlled
employing
performance
liquid
hplc
allows
precise
reversibly
associating
macromolecules
proteins
complex
migrates
faster
components
difference
elution
profile
mixture
summation
profiles
provides
quantifiable
indication
degree
molecular
delta
qualitatively
reveal
presence
absence
significant
rank
relative
comparing
samples
combination
computer
simulation
quantify
magnitude
arrangement
coupled
analytical
instrumentation
novel
manner
performance liquid
liquid chromatograph
computer controlled
liquid chromatography
computer control
size-exclusion chromatography
analytical instrument
computer simulation
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