pLS010 plasmid vector

Patent · Fri Jan 01 00:00:00 EST 1988

OSTI ID:866521

Lacks, Sanford A ^[1]; Balganesh, Tanjore S ^[2]

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1. Brookhaven, NY
2. Upton, NY

Disclosed is recombinant plasmid pLS101, consisting essentially of a 2.0 Kb malM gene fragment ligated to a 4.4 Kb T.sub.c r DNA fragment, which is particularly useful for transforming Gram-positive bacteria. This plasmid contains at least four restriction sites suitable for inserting exogeneous gene sequences. Also disclosed is a method for plasmid isolation by penicillin selection, as well as processes for enrichment of recombinant plasmids in Gram-positive bacterial systems.

• Research Organization: Associated Universities, Inc., Upton, NY (United States)
• DOE Contract Number: AC02-76CH00016
• Assignee: United States of America as represented by United States (Washington, DC)
• Patent Number(s): US 4729954
• OSTI ID: 866521
• Country of Publication: United States
• Language: English

Related Subjects

pls010
plasmid
vector
disclosed
recombinant
pls101
consisting
essentially
kb
malm
fragment
ligated
dna
particularly
useful
transforming
gram-positive
bacteria
contains
restriction
sites
suitable
inserting
exogeneous
sequences
method
isolation
penicillin
selection
processes
enrichment
plasmids
bacterial
systems
consisting essentially
particularly useful
dna fragment
restriction site
recombinant plasmids
recombinant plasmid
gram-positive bacteria
plasmid contains
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