Automated apparatus for producing gradient gels

Patent · Wed Jan 01 00:00:00 EST 1986

OSTI ID:865874

Anderson, Norman L ^[1]

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1. Clarendon Hills, IL

Apparatus for producing a gradient gel which serves as a standard medium for a two-dimensional analysis of proteins, the gel having a density gradient along its height formed by a variation in gel composition, with the apparatus including first and second pumping means each including a plurality of pumps on a common shaft and driven by a stepping motor capable of providing small incremental changes in pump outputs for the gel ingredients, the motors being controlled, by digital signals from a digital computer, a hollow form or cassette for receiving the gel composition, means for transferring the gel composition including a filler tube extending near the bottom of the cassette, adjustable horizontal and vertical arms for automatically removing and relocating the filler tube in the next cassette, and a digital computer programmed to automatically control the stepping motors, arm movements, and associated sensing operations involving the filling operation.

• Research Organization: Argonne National Laboratory (ANL), Argonne, IL (United States)
• DOE Contract Number: W-31109-ENG-38
• Assignee: United States of America as represented by United States ()
• Patent Number(s): US 4594064
• OSTI ID: 865874
• Country of Publication: United States
• Language: English

Related Subjects

automated
apparatus
producing
gradient
gels
serves
standard
medium
two-dimensional
analysis
proteins
density
height
formed
variation
composition
including
pumping
means
plurality
pumps
common
shaft
driven
stepping
motor
capable
providing
incremental
changes
pump
outputs
ingredients
motors
controlled
digital
signals
computer
hollow
form
cassette
receiving
transferring
filler
tube
extending
near
bottom
adjustable
horizontal
vertical
arms
automatically
removing
relocating
programmed
control
arm
movements
associated
sensing
operations
involving
filling
operation
stepping motor
digital signals
pumping means
digital signal
apparatus including
digital computer
density gradient
tube extending
composition including
automatically control
automated apparatus
motor capable
common shaft
/425/204/