Enhanced growth medium and method for culturing human mammary epithelial cells

Patent · Sat Jan 01 00:00:00 EST 1983

OSTI ID:864822

Stampfer, Martha R ^[1]; Smith, Helene S ^[2]; Hackett, Adeline J ^[3]

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1. 7290 Sayre Dr., Oakland, CA 94611
2. 5693 Cabot Dr., Oakland, CA 94611
3. 82 Evergreen Dr., Orinda, CA 94563

Methods are disclosed for isolating and culturing human mammary epithelial cells of both normal and malignant origin. Tissue samples are digested with a mixture including the enzymes collagenase and hyaluronidase to produce clumps of cells substantially free from stroma and other undesired cellular material. Growing the clumps of cells in mass culture in an enriched medium containing particular growth factors allows for active cell proliferation and subculture. Clonal culture having plating efficiencies of up to 40% or greater may be obtained using individual cells derived from the mass culture by plating the cells on appropriate substrates in the enriched media. The clonal growth of cells so obtained is suitable for a quantitative assessment of the cytotoxicity of particular treatment. An exemplary assay for assessing the cytotoxicity of the drug adriamycin is presented.

• Research Organization: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
• Assignee: Stampfer, Martha R. (7290 Sayre Dr., Oakland, CA 94611);Smith, Helene S. (5693 Cabot Dr., Oakland, CA 94611);Hackett, Adeline J. (82 Evergreen Dr., Orinda, CA 94563)
• Patent Number(s): US 4423145
• OSTI ID: 864822
• Country of Publication: United States
• Language: English

Related Subjects

enhanced
growth
medium
method
culturing
human
mammary
epithelial
cells
methods
disclosed
isolating
normal
malignant
origin
tissue
samples
digested
mixture
including
enzymes
collagenase
hyaluronidase
produce
clumps
substantially
free
stroma
undesired
cellular
material
growing
mass
culture
enriched
containing
particular
factors
allows
active
cell
proliferation
subculture
clonal
plating
efficiencies
40
obtained
individual
derived
appropriate
substrates
media
suitable
quantitative
assessment
cytotoxicity
treatment
exemplary
assay
assessing
drug
adriamycin
cellular material
tissue sample
individual cells
substantially free
individual cell
medium containing
mixture including
tissue samples
growth medium
epithelial cells
human mammary
mammary epithelial
active cell
culturing human
/435/