Fluidic assembly for an ultra-high-speed chromosome flow sorter

Patent · Fri Jan 01 00:00:00 EST 1982

OSTI ID:864421

Gray, Joe W ^[1]; Alger, Terry W ^[1]; Lord, David E ^[1]

---

1. Livermore, CA

A fluidic assembly for an ultra-high-speed chromosome flow sorter using a fluid drive system, a nozzle with an orifice having a small ratio of length to diameter, and mechanism for vibrating the nozzle along its axis at high frequencies. The orifice is provided with a sharp edge at its inlet, and a conical section at its outlet for a transition from a short cylindrical aperture of small length to diameter ratio to free space. Sample and sheath fluids in separate low pressure reservoirs are transferred into separate high pressure buffer reservoirs through a valve arrangement which first permit the fluids to be loaded into the buffer reservoirs under low pressure. Once loaded, the buffer reservoirs are subjected to high pressure and valves are operated to permit the buffer reservoirs to be emptied through the nozzle under high pressure. A sensor and decision logic is positioned at the exit of the nozzle, and a charging pulse is applied to the jet when a particle reaches a position further downstream where the droplets are formed. In order to adjust the timing of charge pulses, the distance between the sensing station at the outlet of the nozzle and the droplet breakoff point is determined by stroboscopic illumination of the droplet breakoff region using a laser and a revolving lucite cylinder, and a beam on/off modulator. The breakoff point in the region thus illuminated may then be viewed, using a television monitor.

• Research Organization: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
• DOE Contract Number: W-7405-ENG-48
• Assignee: United States of America as represented by United States (Washington, DC)
• Patent Number(s): US 4361400
• OSTI ID: 864421
• Country of Publication: United States
• Language: English

Related Subjects

fluidic
assembly
ultra-high-speed
chromosome
flow
sorter
fluid
drive
nozzle
orifice
ratio
length
diameter
mechanism
vibrating
axis
frequencies
provided
sharp
edge
inlet
conical
section
outlet
transition
cylindrical
aperture
free
space
sample
sheath
fluids
separate
pressure
reservoirs
transferred
buffer
valve
arrangement
permit
loaded
subjected
valves
operated
emptied
sensor
decision
logic
positioned
exit
charging
pulse
applied
jet
particle
reaches
position
downstream
droplets
formed
adjust
timing
charge
pulses
distance
sensing
station
droplet
breakoff
determined
stroboscopic
illumination
region
laser
revolving
lucite
cylinder
beam
modulator
illuminated
viewed
television
monitor
charge pulses
sheath fluid
free space
diameter ratio
valve arrangement
television monitor
sharp edge
chromosome flow
ultra-high-speed chromosome
fluidic assembly
flow sorter
fluid drive
decision logic
/256/209/239/