Genetic Engineering of a Radiation Resistant Bacterium for Biodegradation of Mixed Wastes
The specific aims of this project are to: (1) Clone and express broad spectrum oxygenases in D. radiodurans with a target TCE degradation rate of 1 nmol/min/mg protein, (2) analyze and upregulate stress response systems in D. radiodurans, and (3) test survival and activities of these strains in artificial mixtures of contaminants, designed to simulate DOE mixed waste streams, using bench-scale treatment reactors. In addition, we proposed to generate a set of new genetic tools to carry out this work. In this report, progress is described in the development of new tools and in the study of solvent resistance, heat shock regulation, and polyP metabolism.
- Research Organization:
- University of Washington, Seattle, WA (US)
- Sponsoring Organization:
- USDOE Office of Science (SC) (US)
- DOE Contract Number:
- FG07-97ER20294
- OSTI ID:
- 834617
- Report Number(s):
- EMSP-73833-2003; R&D Project: EMSP 73833; TRN: US200433%%241
- Resource Relation:
- Other Information: PBD: 1 Jun 2003
- Country of Publication:
- United States
- Language:
- English
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