Synchrotron-based FTIR spectromicroscopy: Cytotoxicity and heating considerations
Synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectromicroscopy is a newly emerging bioanalytical and imaging tool. This unique technique provides mid-infrared (IR) spectra, hence chemical information, with high signal-to-noise at spatial resolutions as fine as 3 to 10 microns. Thus it enables researchers to locate, identify, and track specific chemical events within an individual living mammalian cell. Mid-IR photons are too low in energy (0.05 - 0.5 eV) to either break bonds or to cause ionization. In this review, we show that the synchrotron IR beam has no detectable effects on the short- and long-term viability, reproductive integrity, cell-cycle progression, and mitochondrial metabolism in living human cells, and produces only minimal sample heating (< 0.5 degrees C). These studies have established an important foundation for SR-FTIR spectromicroscopy in biological and biomedical research.
- Research Organization:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Organization:
- USDOE Director, Office of Science. Office of Basic Energy Sciences. Materials Science and Engineering Division (US)
- DOE Contract Number:
- AC03-76SF00098
- OSTI ID:
- 816214
- Report Number(s):
- LBNL-51900; R&D Project: G40001; TRN: US0304940
- Journal Information:
- Journal of Biological Physics, Vol. 29, Issue 2/3; Other Information: Journal Publication Date: 2003; PBD: 13 Dec 2002
- Country of Publication:
- United States
- Language:
- English
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Tracking chemical changes in a live cell: Biomedical applications of SR-FTIR spectromicroscopy
Synchrotron infrared spectromicroscopy as a novel bioanalytical microprobe for individual living cells: Cytotoxicity considerations
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
54 ENVIRONMENTAL SCIENCES
ANIMAL CELLS
CELL CYCLE
HEATING
IONIZATION
METABOLISM
PHOTONS
SPATIAL RESOLUTION
SPECTRA
SYNCHROTRONS
VIABILITY
SYNCHROTRON FTIR SPECTROMICROSCOPY HEATING CYTOTOXICITY VIABILITY ALCIAN BLUE COLONY FORMATION BRDU MTT.