Modified cellulose synthase gene from 'Arabidopsis thaliana' confers herbicide resistance to plants
Cellulose synthase ('CS'), a key enzyme in the biosynthesis of cellulose in plants is inhibited by herbicides comprising thiazolidinones such as 5-tert-butyl-carbamoyloxy-3-(3-trifluromethyl) phenyl-4-thiazolidinone (TZ), isoxaben and 2,6-dichlorobenzonitrile (DCB). Two mutant genes encoding isoxaben and TZ-resistant cellulose synthase have been isolated from isoxaben and TZ-resistant Arabidopsis thaliana mutants. When compared with the gene coding for isoxaben or TZ-sensitive cellulose synthase, one of the resistant CS genes contains a point mutation, wherein glycine residue 998 is replaced by an aspartic acid. The other resistant mutation is due to a threonine to isoleucine change at amino acid residue 942. The mutant CS gene can be used to impart herbicide resistance to a plant; thereby permitting the utilization of the herbicide as a single application at a concentration which ensures the complete or substantially complete killing of weeds, while leaving the transgenic crop plant essentially undamaged.
- Research Organization:
- Carnegie Institute of Washington (US)
- Sponsoring Organization:
- US Department of Energy (US)
- DOE Contract Number:
- FG02-94ER20133
- Assignee:
- CHO
- Patent Number(s):
- PATENTS-US-A9686234
- Application Number:
- 9-686,234; TRN: US200306%%346
- OSTI ID:
- 808234
- Resource Relation:
- Patent File Date: 2000 Oct 11; Other Information: PBD: 11 Oct 2000
- Country of Publication:
- United States
- Language:
- English
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