[DNA sample manipulation and automation.] Final report
Goals for the project were the following: (1) Development of 1536 well based DNA sequencing platform, (2) Integration of the above device into the CuraGen MicroNiagara system, PerSeptive BioSystems Voyager MALDI Mass Spec and validation of results from the ABI 377 DNA sequencer, (3) Collaboration with the DOE Joint Genome Institute (JGI) to build Sequatron-like systems for DNA purification and DNA sequencing, and working with JGI to implement 1536 well system if required, and (4) Demonstration of the use of robotic systems through genomic sequencing of up to 1Mb DNA. Emphasis over the past year was on the following: DNA purification methods, module development (1536 well thermal cycling; Mass Spectrometry, 96 lane development & Automated gel loading; 96 lane ABI system; MALDI TOF mass spectrometry; automated gel loading system for the ABI), and helping the JGI with Sequatron-like systems.
- Research Organization:
- University of Florida, Gainesville, FL (US)
- Sponsoring Organization:
- USDOE Office of Science (US)
- DOE Contract Number:
- FG02-98ER62623
- OSTI ID:
- 808070
- Report Number(s):
- TWO; TRN: US200716%%497
- Resource Relation:
- Other Information: PBD: 13 Sep 2001
- Country of Publication:
- United States
- Language:
- English
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