High speed DNA sequence analysis by matrix-assisted laser desorption mass spectrometry. Final report for period February 15, 1991 - February 14, 2001
This grant had as its focus (i) to develop chemistry and enzymology to permit the enzymatic synthesis of 2' fluoro modified Sanger sequencing reactions, which would be resistant to fragmentation during MALDI process, (ii) to develop rapid MALDI analyses of DNA sequence polymorphisms using Peptide Nucleic Acid (PNA) DNA analogs in conjunction with solid phase chemistry (iii) to study the fundamental mechanisms occurring in the MALDI analysis of nucleic acids.
- Research Organization:
- University of Wisconsin-Madison, Madison, WI (US)
- Sponsoring Organization:
- US Department of Energy (US)
- DOE Contract Number:
- FG02-91ER61130
- OSTI ID:
- 798154
- Report Number(s):
- DOE/ER/61130-10; TRN: US200308%%210
- Resource Relation:
- Other Information: PBD: 17 Apr 2001; PBD: 17 Apr 2001
- Country of Publication:
- United States
- Language:
- English
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