Library Resources for Bac End Sequencing. Final Technical Report
Studies directed towards the specific aims outlined for this research award are summarized. The RPCI II Human Bac Library has been expanded by the addition of 6.9-fold genomic coverage. This segment has been generated from a MBOI partial digest of the same anonymous donor DNA used for the rest of the library. A new cloning vector, pTARBAC1, has been constructed and used in the construction of RPCI-II segment 5. This new cloning vector provides a new strategy in identifying targeted genomic regions and will greatly facilitate a large-scale analysis for positional cloning. A new maleCS7BC/6J mouse BAC library has been constructed. RPCI-23 contain 576 plates (approx 210,000 clones) and represents approximately 11-fold coverage of the mouse genome.
- Research Organization:
- Health Research Inc., Buffalo, NY (US)
- Sponsoring Organization:
- US Department of Energy (US)
- DOE Contract Number:
- FG02-98ER62675
- OSTI ID:
- 772661
- Resource Relation:
- Other Information: PBD: 1 Oct 2000
- Country of Publication:
- United States
- Language:
- English
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