Final report. Human artificial episomal chromosome (HAEC) for building large genomic libraries
Collections of human DNA fragments are maintained for research purposes as clones in bacterial host cells. However for unknown reasons, some regions of the human genome appear to be unclonable or unstable in bacteria. Their team has developed a system using episomes (extrachromosomal, autonomously replication DNA) that maintains large DNA fragments in human cells. This human artificial episomal chromosomal (HAEC) system may prove useful for coverage of these especially difficult regions. In the broader biomedical community, the HAEC system also shows promise for use in functional genomics and gene therapy. Recent improvements to the HAEC system and its application to mapping, sequencing, and functionally studying human and mouse DNA are summarized. Mapping and sequencing the human genome and model organisms are only the first steps in determining the function of various genetic units critical for gene regulation, DNA replication, chromatin packaging, chromosomal stability, and chromatid segregation. Such studies will require the ability to transfer and manipulate entire functional units into mammalian cells.
- Research Organization:
- University of North Carolina at Chapel Hill, Chapel Hill, NC (US)
- Sponsoring Organization:
- USDOE Office of Energy Research (ER) (US)
- DOE Contract Number:
- FG05-91ER61135
- OSTI ID:
- 764441
- Report Number(s):
- DOE/ER/61135-1-Vol.1; TRN: AH200103%%321
- Resource Relation:
- Other Information: PBD: 9 Dec 1999
- Country of Publication:
- United States
- Language:
- English
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