skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Final Technical Report. Origins of subsurface microorganisms: Relating laboratory microcosm studies to a geologic time scale

Technical Report ·
DOI:https://doi.org/10.2172/761931· OSTI ID:761931

This project was conducted as part of the Department of Energy's Deep Subsurface Science Program. It was part of a larger effort to determine the origins of subsurface microorganisms. Two hypotheses have been suggested for the origins of subsurface microorganisms: (1) microorganisms were deposited at the time of (or shortly after) geologic deposition of rocks and sediments (the in situ survival hypothesis), and (2) microorganisms have been transported from surface environments to subsurface rocks and sediments since the time of geologic deposition (transport hypothesis). These two hypotheses are not mutually exclusive. Depending on the geological setting, either one or both of these hypotheses may best explain microbial origins. Our project focused on the in situ survival hypothesis. We tested the hypothesis that microorganisms (individuals populations and communities) can survive long-term sequestration within subsurface sediments. Other objectives were to identify geologic conditions that favor long-term survival, identify physiological traits of microorganisms that favor long-term survival, and determine which groups of microorganisms are most likely to survive long-term sequestration in subsurface sediments. We tested this hypothesis using a combination of pure culture techniques in laboratory microcosms under controlled conditions and field experiments with buried subsurface sediments.

Research Organization:
New Mexico Institute of Mining and Technology, Socorro, NM (US); University of Nevada, Department of Biological Sciences, Las Vegas, NV (US)
Sponsoring Organization:
USDOE Office of Energy Research (ER) (US)
DOE Contract Number:
FG03-93ER61683
OSTI ID:
761931
Resource Relation:
Other Information: PBD: 1 Dec 1998
Country of Publication:
United States
Language:
English