CLOSTRIDIUM SPORE ATTACHMENT TO HUMAN CELLS
This paper uses high resolution scanning electron microscopy (SEM) with a LaB6 gun and the newest commercial field emission guns, to obtain high magnification images of intact clostridial spores throughout the activation/germination/outgrowth process. By high resolution SEM, the clostridial exosporial membrane can be seen to produce numerous delicate projections (following activation), that extend from the exosporial surface to a nutritive substrate (agar), or cell surface when anaerobically incubated in the presence of human cells (embryonic fibroblasts and colon carcinoma cells). Magnifications of 20,000 to 200,000Xs at accelerating voltages low enough to minimize or eliminate specimen damage (1--5 kV) have permitted the entire surface of C.sporogenes and C.difficile endospores to be examined during all stages of germination. The relationships between the spore and the agar or human cell surface were also clearly visible.
- Research Organization:
- Brookhaven National Lab. (BNL), Upton, NY (United States)
- Sponsoring Organization:
- USDOE Office of Energy Research (ER) (US)
- DOE Contract Number:
- AC02-98CH10886
- OSTI ID:
- 761001
- Report Number(s):
- BNL-67523; KA0404; R&D Project: PO24; KA0404; TRN: AH200035%%75
- Resource Relation:
- Conference: MICROSCOPY AND MICROANALYSIS CONFERENCE, CLEVELAND, OH (US), 08/10/1997--08/14/1997; Other Information: PBD: 10 Aug 1997
- Country of Publication:
- United States
- Language:
- English
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