HIV transcription is induced in dying cells

Technical Report · Thu Jun 01 00:00:00 EDT 1995

DOI:https://doi.org/10.2172/74117· OSTI ID:74117

Woloschak, G E; Chang-Liu, Chin-Mei ^[1]; Schreck, S ^[1]

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1. Argonne National Lab., IL (United States)

Using HeLa cells stably transfected with an HIV-LTR-CAT construct, we demonstrated a peak in CAT induction that occurs in viable (but not necessarily cell-division-competent) cells 24 h following exposure to some cell-killing agents. {gamma} rays were the only cell-killing agent which did not induce HIV transcription; this can be attributed to the fact that {gamma}-ray-induced apoptotic death requires functional p53, which is not present in HeLa cells. For all other agents, HIV-LTR induction was dose-dependent and correlated with the amount of cell killing that occurred in the culture. 14 refs., 4 figs., 1 tab.

• Research Organization: Argonne National Lab. (ANL), Argonne, IL (United States)
• Sponsoring Organization: USDOE, Washington, DC (United States)
• DOE Contract Number: W-31109-ENG-38
• OSTI ID: 74117
• Report Number(s): ANL/CMB/PP-85889; ON: DE95012211; TRN: 95:004901
• Resource Relation: Other Information: PBD: [1995]
• Country of Publication: United States
• Language: English

Related Subjects

56 BIOLOGY AND MEDICINE
APPLIED STUDIES
55 BIOLOGY AND MEDICINE
BASIC STUDIES
VIRUSES
TRANSCRIPTION
HELA CELLS
CELL KILLING
GAMMA RADIATION
GENETIC RADIATION EFFECTS
MAN
IMMUNE SYSTEM DISEASES
CHLORAMPHENICOL
RADIATIONS
CELL DIVISION
GENES
TRANSFERASES
DNA