Single molecule detection using charge-coupled device array technology
A technique for the detection of single fluorescent chromophores in a flowing stream is under development. This capability is an integral facet of a rapid DNA sequencing scheme currently being developed by Los Alamos National Laboratory. In previous investigations, the detection sensitivity was limited by the background Raman emission from the water solvent. A detection scheme based on a novel mode of operating a Charge-Coupled Device (CCD) is being developed which should greatly enhance the discrimination between fluorescence from a single molecule and the background Raman scattering from the solvent. Register shifts between rows in the CCD are synchronized with the sample flow velocity so that fluorescence from a single molecule is collected in a single moving charge packet occupying an area approaching that of a single pixel while the background is spread evenly among a large number of pixels. Feasibility calculations indicate that single molecule detection should be achieved with an excellent signal-to-noise ratio.
- Research Organization:
- Arizona Univ., Tucson, AZ (United States). Dept. of Chemistry
- Sponsoring Organization:
- USDOE; USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG02-90ER60986
- OSTI ID:
- 7237575
- Report Number(s):
- DOE/ER/60986-T1; ON: DE92019242
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
47 OTHER INSTRUMENTATION
59 BASIC BIOLOGICAL SCIENCES
MOLECULES
DETECTION
NUCLEOTIDES
FLUORESCENCE
CHARGE-COUPLED DEVICES
DNA SEQUENCING
LASERS
PROGRESS REPORT
RAMAN EFFECT
SIGNAL-TO-NOISE RATIO
WATER
DOCUMENT TYPES
HYDROGEN COMPOUNDS
LUMINESCENCE
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
SEMICONDUCTOR DEVICES
STRUCTURAL CHEMICAL ANALYSIS
400102* - Chemical & Spectral Procedures
440600 - Optical Instrumentation- (1990-)
550300 - Cytology