Isolation and partial sequence of the A-protein gene of Thermus thermophilus cytochrome c/sub 1/aa/sub 3/
Thermus thermophilus is a strictly aerobic eubacterium which grows optimally near 70/degree/C. Its respiratory system is very similar to that of eukaryotic mitochondria, and the organism has proven to be a particularly good source of stable, comparatively simple respiratory enzymes. There are at least two terminal oxidases: The recently discovered cytochrome ba/sub 3//sup 3/ and cytochrome c/sub 1/aa/sub 3//sup 2/. Cytochrome ba/sub 3/ is analog of aa/sub 3/ in which the heme A of cytochrome a is replaced with protoporphyrin IX (heme B) while its order redox components appear to be largely identical to those of the now classical mammalian cytochrome aa/sub 3/; it has only a single 35 kD protein subunit. Cytochrome c/sub 1/aa/sub 3/ consists of two polypeptides. The /approximately/33 kD C-protein covalently binds one heme C, while the /approximately/55 kD protein is thought to bind the four canonical redox centers of aa/sub 3/, two heme A, and two Cu. Toward our goal of unequivocally establishing the distribution of the metal centers in cytochrome c/sub 1/aa/sub 3/, we have isolated the structural gene of the A-protein. 20 refs., 4 figs.
- Research Organization:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- DOE Contract Number:
- W-7405-ENG-36
- OSTI ID:
- 7095979
- Report Number(s):
- LA-UR-88-2954; CONF-8806246-1; ON: DE89000374
- Resource Relation:
- Conference: Conference on cytochrome oxidase: structure, function, and physiopathology, Rome, Italy, 20 Jun 1988; Other Information: Portions of this document are illegible in microfiche products
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BIOCHEMISTRY
EVALUATION
MITOCHONDRIA
SIMULATION
THERMOPHILIC CONDITIONS
TOLERANCE
AMINO ACIDS
BACTERIA
BENDING
CYTOCHROME OXIDASE
ENZYMES
RESPIRATORY SYSTEM
TEMPERATURE DEPENDENCE
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMISTRY
HAEM DEHYDROGENASES
MICROORGANISMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
OXIDOREDUCTASES
550200* - Biochemistry