Study of the Mn-binding sites in photosystem II using antibodies raised against lumenal regions of the D1 and D2 reaction center proteins
The experiments discussed in this thesis focus on identifying the protein segments or specific amino acids which provide ligands to the Mn cluster of photosystem II (PS II). This Mn cluster plays a central role in the oxygen-evolving complex (OEC) of PS II. The Mn cluster is thought to be bound by lumenal regions of the PS II reaction center proteins known as D1 and D2. First, several peptides were synthesized which correspond to specific lumenal segments of the D1 and D2 proteins. Next, polyclonal antibodies were successfully elicited using three of these peptides. The peptides recognized by these antibodies correspond to protein segments of the spinach reaction center proteins: Ile-321 to Ala-344 of D1 (D1-a), Asp-319 to Arg-334 of D1 (D1-b), and Val-300 to Asn-319 of D2 (D2-a). These antibodies were then used in assays which were developed to structurally or functionally probe the potential Mn-binding regions of the D1 and D2 proteins.
- Research Organization:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Organization:
- USDOE; USDOE, Washington, DC (United States)
- DOE Contract Number:
- AC03-76SF00098
- OSTI ID:
- 7045147
- Report Number(s):
- LBL-32361; ON: DE92017103
- Resource Relation:
- Other Information: Thesis (Ph.D.)
- Country of Publication:
- United States
- Language:
- English
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14 SOLAR ENERGY
OXYGEN
GAS YIELDS
PHOTOSYNTHETIC MEMBRANES
PHOTOCHEMICAL REACTIONS
SPINACH
PHOTOSYNTHETIC REACTION CENTERS
ANTIBODIES
BIOASSAY
MANGANESE COMPLEXES
PEPTIDES
PROTEIN STRUCTURE
CHEMICAL REACTIONS
COMPLEXES
ELEMENTS
FOOD
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MEMBRANES
NONMETALS
ORGANIC COMPOUNDS
PLANTS
PROTEINS
TRANSITION ELEMENT COMPLEXES
VEGETABLES
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