Direct assay of radiation-induced DNA base lesions in mammalian cells
Adenine (Ade), 2'-deoxyadenosine (dAdo), 5'-deoxyadenosine monophosphate (dAUT), single stranded poly adenylic acid [poly (dA)], double stranded deoxyadenylic-thymidylic acid [ds poly (dA-T)] and salmon testis DNA were irradiated with 500 Gy under oxic and anoxic conditions. The major damage products were analyzed by BPLC with optical detection and quantitated in terms of the percentage of the adenosine in each model compound found as a specific damage product. Outside of the Ade free base, 8-OH-dAdo was the major oxic damage product from each model compound. The type and quantity of the major damage products depended on the sequence and conformation of the model compounds under anoxic conditions. When dAdo and dAMP were irradiated under anoxic conditions, the major damage product was either the R or S isomer of 8,5'cdAdo and little Ade or [alpha]-dAdo was observed. However, when poly(dA), poly(dA-dT), and salmon testis DNA were [gamma]-irradiated under nitrogen, the major deoxyadenosine damage product was identified as the [alpha]-anomer of deoxyadenosine. No [alpha]-deoxyadenosine was detected after irradiation under oxic conditions. The presence of nucleotides with the [alpha]-configuration at the anomeric carbon atom in the DNA chain may have a significant effect on its tertiary structure and possibly modify its biological activity.
- Research Organization:
- Bowman Gray School of Medicine, Winston-Salem, NC (United States)
- Sponsoring Organization:
- USDOE; USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG05-86ER60464
- OSTI ID:
- 7013706
- Report Number(s):
- DOE/ER/60464-6; ON: DE93001832; TRN: 92-038163
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DNA
RADIOBIOLOGY
ANIMAL CELLS
ANOXIA
DNA REPAIR
DNA SEQUENCING
LIQUID COLUMN CHROMATOGRAPHY
NUCLEOTIDES
PROGRESS REPORT
SUPEROXIDE DISMUTASE
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOLOGY
CHROMATOGRAPHY
DOCUMENT TYPES
ENZYMES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PROTEINS
REPAIR
SEPARATION PROCESSES
STRUCTURAL CHEMICAL ANALYSIS
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture