ROAM mutations causing increased expression of yeast genes: their activation by signals directed toward conjugation functions and their formation by insertion of tyl repetitive elements
Mechanisms available to eukaryotic organisms for the coordinate regulation of gene expression are being examined by genetic and biochemical characterization of an unusual mutation, CYC7-H2, which causes overproduction of iso-2-cytochrome c in the yeast Saccharomyces cerevisiae. The CYC7-H2 mutation causes approximately a twenty fold overproduction of iso-2-cytochrome c in haploid strains but only a one to four fold overproduction in MATa/MAT..cap alpha.. diploid strains. This regulation of overproduction has been characterized as a response to signals controlling conjugation in yeast. The CYC7-H2 mutation is closely related to other regulatory mutations occurring at the cargA, cargB and DUR1,2 loci which are the structural genes for arginase, ornithine transaminase and urea amidolyase, respectively. Similar to the CYC7-H2 mutation, the mutations designated cargA/sup +/O/sup h/, cargB/sup +/O/sup h/ and durO/sup h/ cause constitutive production of their respective gene products at much lower levels in MATa/MAT..cap alpha.. diploid strains than in the corresponding haploid strains. Observations characterizing the regulation of overproduction in the CYC7-H2 mutant are presented with the additional and parallel observations for the O/sup h/ mutants.
- Research Organization:
- Rochester Univ., NY (USA). School of Medicine and Dentistry
- DOE Contract Number:
- AC02-76EV03490
- OSTI ID:
- 6945988
- Report Number(s):
- UR-3490-1879; CONF-800595-3
- Resource Relation:
- Conference: Cold Spring Harbor symposium No. 45: movable genetic elements, Cold Spring Harbor, NY, USA, 28 May 1980
- Country of Publication:
- United States
- Language:
- English
Similar Records
Dependence on mating type for the overproduction of iso-2-cytochrome c in the yeast mutant CYC7-H2
Mating signals control expression of mutations resulting from insertion of a transposable repetitive element adjacent to diverse yeast genes