Yeast mutants overproducing iso-cytochromes c
For over 15 years, the iso-cytochrome c system in the yeast Saccharomyces cerevisiae has been used to investigate a multitude of problems in genetics and molecular biology. More recently, attention has been focused on using mutants for examining translation and transcriptional processes and for probing regulatory regions governing gene expression. In an effort to explore regulatory mechanisms and to investigate mutational alterations that lead to increased levels of gene products, we have isolated and characterized mutants that overproduce cytochrome c. In this paper we have briefly summarized background information of some essential features of the iso-cytochrome c system and we have described the types of mutants that overproduce iso-1-cytochrome c or iso-2-cytochrome c. Genetic procedures and recombinant DNA procedures were used to demonstrate that abnormally high amounts of gene products occur in mutants as result of duplications of gene copies or of extended alteration of regulatory regions. The results summarized in this paper point out the requirements of gross mutational changes or rearrangements of chromosomal segments for augmenting gene products.
- Research Organization:
- Rochester Univ., NY (USA). School of Medicine and Dentistry
- DOE Contract Number:
- AC02-76EV03490
- OSTI ID:
- 6926497
- Report Number(s):
- UR-3490-1874; CONF-8006132-1
- Resource Relation:
- Conference: Alfred Benzon symposium 16-molecular genetics in yeast, Copenhagen, Denmark, 15 Jun 1980
- Country of Publication:
- United States
- Language:
- English
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