JANUS neutron irradiation of a mouse cell line containing a shuttle vector plasmid

Conference · Sun Jan 01 00:00:00 EST 1989

OSTI ID:6906083

Nagy, B ^[1]; Grdina, D J ^[2]; Ashman, C R ^[3]

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1. Central Inst. for Tumors, Zagreb (Yugoslavia)
2. Argonne National Lab., IL (USA)
3. Chicago Univ., IL (USA)

The study presented here represents the initial steps of our attempt to characterize JANUS neutron induced mutagenesis in mammalian cells. The approach which we are taking is to use a mammalian cell system which allows one to determine the actual changes in DNA base sequence which occur when a gene mutates. Recently, several systems have been described which make possible the rapid and unambiguous determination of DNA base sequence changes in genes of eukaryotic cells. In some of these systems, a target gene is introduced into the mammalian cells as part of a shuttle vector which is capable of replication in both mammalian cells and bacteria. In this study we have used such a system for the analysis of neutron-induced mutations in the presence and absence of the radioprotector N-(2-mercaptoethyl)-1,3-diaminopropane, WR1065. 20 refs., 5 figs., 1 tab.

• Research Organization: Argonne National Lab., IL (USA)
• Sponsoring Organization: DOE/ER
• DOE Contract Number: W-31109-ENG-38
• OSTI ID: 6906083
• Report Number(s): CONF-8910367-2; ON: DE90010542; TRN: 90-015655
• Resource Relation: Conference: 3. international conference of anticarcinogens and radiation protection, Dubrovnik (Yugoslavia), 15-21 Oct 1989
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
21 SPECIFIC NUCLEAR REACTORS AND ASSOCIATED PLANTS
ANIMAL CELLS
MUTAGENESIS
FISSION NEUTRONS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL REPAIR
CARCINOGENESIS
CHROMOSOMAL ABERRATIONS
DAMAGE
DNA
DNA SEQUENCING
DOSE EQUIVALENTS
GAMMA RADIATION
GENE MUTATIONS
IRRADIATION
JANUS REACTOR
LET
MICE
ONCOGENIC TRANSFORMATIONS
PLASMIDS
RADIATION PROTECTION
TOXICITY
VARIATIONS
ANIMALS
BARYONS
BIOLOGICAL RECOVERY
CELL CONSTITUENTS
CELL TRANSFORMATIONS
ELECTROMAGNETIC RADIATION
ELEMENTARY PARTICLES
ENERGY TRANSFER
ENRICHED URANIUM REACTORS
FERMIONS
HADRONS
IONIZING RADIATIONS
MAMMALS
MUTATIONS
NEUTRONS
NUCLEIC ACIDS
NUCLEONS
ORGANIC COMPOUNDS
PATHOGENESIS
RADIATION EFFECTS
RADIATIONS
REACTORS
RECOVERY
REPAIR
RESEARCH AND TEST REACTORS
RESEARCH REACTORS
RODENTS
STRUCTURAL CHEMICAL ANALYSIS
TANK TYPE REACTORS
THERMAL REACTORS
VERTEBRATES
WATER COOLED REACTORS
WATER MODERATED REACTORS
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture
220600 - Nuclear Reactor Technology- Research
Test & Experimental Reactors