Carotenoid biosynthesis in bacteria: In vitro studies of a crt/bch transcription factor from Rhodobacter capsulatus and carotenoid enzymes from Erwinia herbicola
A putative transcription factor in Rhodobactor capsulatus which binds upstream of the crt and bch pigment biosynthesis operons and appears to play a role in the adaptation of the organism from the aerobic to the anaerobic-photosynthetic growth mode was characterized. Chapter 2 describes the identification of this factor through an in vitro mobility shift assay, as well as the determination of its binding properties and sequence specificity. Chapter 3 focuses on the isolation of this factor. Biochemistry of later carotenoid biosynthesis enzymes derived from the non-photosynthetic bacterium, Erwinia herbicola. Chapter 4 describes the separate overexpression and in vitro analysis of two enzymes involved in the main sequence of the carotenoid biosynthesis pathway, lycopene cyclase and 5-carotene hydroxylase. Chapter 5 examines the overexpression and enzymology of functionally active zeaxanthin glucosyltransferase, an enzyme which carries out a more unusual transformation, converting a carotenoid into its more hydrophilic mono- and diglucoside derivatives. In addition, amino acid homology with other glucosyltransferases suggests a putative binding site for the UDP-activated glucose substrate.
- Research Organization:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Organization:
- USDOE; USDOE, Washington, DC (United States)
- DOE Contract Number:
- AC03-76SF00098
- OSTI ID:
- 6880113
- Report Number(s):
- LBL-33496; ON: DE93010409
- Resource Relation:
- Other Information: Thesis (Ph.D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
14 SOLAR ENERGY
CAROTENOIDS
BIOSYNTHESIS
CYCLASES
GENE REGULATION
GENE REPRESSORS
BIOCHEMICAL REACTION KINETICS
GLYCOSYL TRANSFERASES
TRANSCRIPTION FACTORS
PHOTOSYNTHETIC BACTERIA
ENZYMES
HYDROCARBONS
KINETICS
LYASES
ORGANIC COMPOUNDS
PIGMENTS
PROTEINS
REACTION KINETICS
SYNTHESIS
TERPENES
TRANSFERASES
550200* - Biochemistry
550500 - Metabolism
140505 - Solar Energy Conversion- Photochemical
Photobiological
& Thermochemical Conversion- (1980-)