Osmoregulation in methanogens

Technical Report · Fri Jan 01 00:00:00 EST 1993

DOI:https://doi.org/10.2172/6849055· OSTI ID:6849055

Roberts, M F

Our major goal of our work has been to develop and use NMR techniques to study how methanogenic archaebacteria deal with osmotic stress with the hope of providing insights into increasing the salt tolerance of other cells. The project has three main sections: (i) in vivo studies of methanogens; (ii) use of [sup l3]C- and [sup l5]N- labeled potential precursors and in vitro analyses of specific label uptake for elucidation of osmolyte dynamics and biosynthetic pathways of osmolytes in these organisms, and isolation of key biosynthetic enzymes; and (iii) collaborative studies on identification of organic solutes in other methanogens.

• Research Organization: Boston Coll., Chestnut Hill, MA (United States). Merkert Chemistry Center
• Sponsoring Organization: USDOE; USDOE, Washington, DC (United States)
• DOE Contract Number: FG02-91ER20025
• OSTI ID: 6849055
• Report Number(s): DOE/ER/20025-1; ON: DE93013130
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
59 BASIC BIOLOGICAL SCIENCES
METHANOGENIC BACTERIA
BIOLOGICAL ADAPTATION
NUCLEAR MAGNETIC RESONANCE
BIOLOGICAL STRESS
CARBON 13
CELL MEMBRANES
DERIVATIZATION
ISOMERASES
MEMBRANE TRANSPORT
NITROGEN 15
POTASSIUM 39
PROGRESS REPORT
SALINITY
TRACER TECHNIQUES
ALKALI METAL ISOTOPES
BACTERIA
CARBON ISOTOPES
CELL CONSTITUENTS
CHEMICAL REACTIONS
DOCUMENT TYPES
ENZYMES
EVEN-ODD NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MEMBRANES
MICROORGANISMS
NITROGEN ISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
POTASSIUM ISOTOPES
PROTEINS
RESONANCE
STABLE ISOTOPES
090900* - Biomass Fuels- Processing- (1990-)
550700 - Microbiology
550500 - Metabolism