Neutron scattering studies of the H2a-H2b and (H3-H4)/sub 2/ histone complexes
Neutron scattering experiments have shown that both the (H3-H4)/sub 2/ and H2a-H2b histone complexes are quite asymmetric in solution. The (H3-H4)/sub 2/ tetramer is an oblate or flattened structure, with a radius of gyration almost as large as that of the core octamer. If the tetramer is primarily globular, it must have an axial ratio of about 1:5. It is more likely, however, that this asymmetry results in part from N-terminal arms that extend outward approximately within the major plane of the particle. If this is the case, less asymmetric models for the globular part of the tetramer, including a dislocated disk, can be made consistent with the scattering data. The H2a-H2b dimer, on the other hand, is an elongated structure. 48 references, 12 figures, 1 table.
- Research Organization:
- Brookhaven National Lab., Upton, NY (USA)
- DOE Contract Number:
- AC02-76CH00016
- OSTI ID:
- 6808243
- Report Number(s):
- BNL-34674; CONF-8206240-5; ON: DE84012173
- Resource Relation:
- Conference: Brookhaven symposium biology 32, Upton, NY, USA, 1 Jun 1982; Other Information: Portions are illegible in microfiche products
- Country of Publication:
- United States
- Language:
- English
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PHYSICAL AND ANALYTICAL CHEMISTRY
HISTONES
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NUCLEAR MAGNETIC RESONANCE
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HYDROGEN DEUTERIDE
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CHROMATIN
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DEUTERIDES
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MASTIGOPHORA
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MICROORGANISMS
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