DNA sequencing by a single molecule detection of labeled nucleotides sequentially cleaved from a single strand of DNA

Conference · Fri Jan 01 00:00:00 EST 1993

DOI:https://doi.org/10.1117/12.146712· OSTI ID:6698020

Goodwin, P M; Schecker, J A; Wilkerson, C W; Hammond, M L; Ambrose, W P; Jett, J H; Martin, J C; Marrone, B L; Keller, R A ^[1]; Haces, A; Shih, P J; Harding, J D ^[2]

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1. Los Alamos National Lab., NM (United States)
2. Life Technologies, Inc., Gaithersburg, MD (United States)

We are developing a laser-based technique for the rapid sequencing of large DNA fragments (several kb in size) at a rate of 100 to 1000 bases per second. Our approach relies on fluorescent labeling of the bases in a single fragment of DNA, attachment of this labeled DNA fragment to a support, movement of the supported DNA into a flowing sample stream, sequential cleavage of the end nucleotide from the DNA fragment with an exonuclease, and detection of the individual fluorescently labeled bases by laser-induced fluorescence.

• Research Organization: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
• Sponsoring Organization: USDOE; USDOE, Washington, DC (United States)
• DOE Contract Number: W-7405-ENG-36
• OSTI ID: 6698020
• Report Number(s): LA-UR-93-362; CONF-930159-8; ON: DE93007331
• Resource Relation: Journal Volume: 1891; Conference: International Society for Optical Engineering (SPIE) conference, Los Angeles, CA (United States), 16-23 Jan 1993
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DNA SEQUENCING
OPTIMIZATION
OLIGONUCLEOTIDES
FLUORESCENCE SPECTROSCOPY
AUTOMATION
BIOTIN
AZOLES
CARBOXYLIC ACIDS
EMISSION SPECTROSCOPY
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
IMIDAZOLES
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
SPECTROSCOPY
STRUCTURAL CHEMICAL ANALYSIS
VITAMIN B GROUP
VITAMINS
550200* - Biochemistry
550400 - Genetics